ArticleScientific reports2016
Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Innate immune response to Streptococcus suis infection: advances and future challenges.Veterinary research · 2026Review
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- Mono- and co-infections of primary porcine respiratory cells withInfection and immunity · 2026Article
- Application of Bovine Nasal Epithelial Cells as an In Vitro Model for Studying Viral Infection in the Upper Respiratory Tract.Viruses · 2025Article
- Establishment and validation of red fox (vulpes vulpes) airway epithelial cell cultures at the air-liquid-interface.Scientific reports · 2025Article
- Genomic analysis and virulence of human Streptococcus suis serotype 14.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Primary cell culture systems to investigate host-pathogen interactions in bacterial respiratory tract infections of livestock.Frontiers in cellular and infection microbiology · 2025Review
- Article
- The forecasting power of the mucin-microbiome interplay in livestock respiratory diseases.The veterinary quarterly · 2024Article
- Inflammation plays a critical role in damage to the bronchiolar epithelium induced byInfection and immunity · 2023Article
- Role of Metabolic Adaptation ofPathogens (Basel, Switzerland) · 2023Review
- In Vitro Characteristics of Canine Primary Tracheal Epithelial Cells Maintained at an Air-Liquid Interface Compared to In Vivo Morphology.International journal of molecular sciences · 2023Article
- Overcoming the Barrier of the Respiratory Epithelium during Canine Distemper Virus Infection.mBio · 2022Article
- Pyolysin ofFrontiers in immunology · 2022Article
- Article
- Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of Streptococcus suis in a porcine precision-cut lung slice model.Virulence · 2021Article
- The type II histidine triad protein HtpsC facilitates invasion of epithelial cells by highly virulent Streptococcus suis serotype 2.Journal of microbiology (Seoul, Korea) · 2021Article
- Review of the speculative role of co-infections in Streptococcus suis-associated diseases in pigs.Veterinary research · 2021Review
- Article
- The Airway Pathobiome in Complex Respiratory Diseases: A Perspective in Domestic Animals.Frontiers in cellular and infection microbiology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococci may colonize the epithelium in the airways and other entry sites. While local infection often remains asymptomatic, severe or even fatal diseases occur when streptococci become invasive and spread to different sites in the infected host. We have established porcine respiratory air-liquid interface cultures (ALI) from the porcine lung to analyze the interaction of streptococci with their primary target cells. As representative of the streptococcal family we chose Streptococcus suis (S. suis) that is not only a major swine respiratory pathogen but can also infect humans. Suilysin, a cholesterol-dependent cytolysin (CDC), is an important virulence factor. By comparing a S. suis wt strain with a suilysin-deficient mutant, we demonstrate that suilysin contributes to (i) adherence to airway cells (ii) loss of ciliated cells (iii) apoptosis, and (iv) invasion. Furthermore, we show that cytolytic activity of suilysin is crucial for these effects. A striking result of our analysis was the high efficiency of S. suis-induced apoptosis and invasion upon infection under ALI conditions. These properties have been reported to be less efficient when analyzed with immortalized cells. We hypothesize that soluble effectors such as suilysin are present at higher concentrations in cells kept at ALI conditions and thus more effective. These results should be relevant also for infection of the respiratory tract by other respiratory pathogens.
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