ArticlePloS one2016
Two UGT84 Family Glycosyltransferases Catalyze a Critical Reaction of Hydrolyzable Tannin Biosynthesis in Pomegranate (Punica granatum).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- WRKY14-DPB Module Enhances Drought Tolerance by Activating the Expression of UGT84B1 Involved in Hydrolyzable Tannin Biosynthesis.Plant, cell & environment · 2026Article
- Mass Spectrometry Imaging-Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Transcriptomic, biochemical, and microbiome assessments into drought and salinity tolerance in durum wheat mediated by plant growth-promoting bacteria.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Article
- Production of cinnamates and benzoates glucose esters by bioconversion usingPlant biotechnology (Tokyo, Japan) · 2024Article
- Elucidation and reconstitution of hydrolyzable tannin biosynthesis.Plant biotechnology (Tokyo, Japan) · 2024Article
- Identification of MYB Transcription Factor, a Regulator Related to Hydrolysable Tannin Synthesis inPlants (Basel, Switzerland) · 2024Article
- Identification of Key Genes Associated with 1,2,6-Tri-O-galloyl-β-D-glucopyranose Accumulation inFoods (Basel, Switzerland) · 2024Article
- RNA-seq and metabolomic analyses of beneficial plant phenol biochemical pathways in red alder.Frontiers in plant science · 2024Article
- Phenological Variations in the Content of Polyphenols and Triterpenoids inPlants (Basel, Switzerland) · 2023Article
- The complexities of proanthocyanidin biosynthesis and its regulation in plants.Plant communications · 2023Review
- Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins.Plants (Basel, Switzerland) · 2022Review
- Article
- Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products.Synthetic and systems biotechnology · 2022Article
- Regulation of Plant Tannin Synthesis in Crop Species.Frontiers in genetics · 2022Review
- Article
- Identification and Characterization of Glucosyltransferase That Forms 1-Galloyl-Molecules (Basel, Switzerland) · 2021Article
- Metabolic changes in pomegranate fruit skin following cold storage promote chilling injury of the peel.Scientific reports · 2021Article
- Two UGT84A Family Glycosyltransferases Regulate Phenol, Flavonoid, and Tannin Metabolism inFrontiers in plant science · 2021Article
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrolyzable tannins (HTs) play important roles in plant herbivore deterrence and promotion of human health. A critical step in HT production is the formation of 1-O-galloyl-β-D-glucopyranoside (β-glucogallin, ester-linked gallic acid and glucose) by a UDP-glucosyltransferase (UGT) activity. We cloned and biochemically characterized four candidate UGTs from pomegranate (Punica granatum), of which only UGT84A23 and UGT84A24 exhibited β-glucogallin forming activities in enzyme assays. Although overexpression and single RNAi knockdown pomegranate hairy root lines of UGT84A23 or UGT84A24 did not lead to obvious alterations in punicalagin (the prevalent HT in pomegranate) accumulation, double knockdown lines of the two UGTs resulted in largely reduced levels of punicalagins and bis-hexahydroxydiphenyl glucose isomers. An unexpected accumulation of galloyl glucosides (ether-linked gallic acid and glucose) was also detected in the double knockdown lines, suggesting that gallic acid was utilized by an unidentified UGT activity for glucoside formation. Transient expression in Nicotiana benthamiana leaves and immunogold labeling in roots of pomegranate seedlings collectively indicated cytosolic localization of UGT84A23 and UGT84A24. Overall, functional characterization and localization of UGT84A23 and UGT84A24 open up opportunities for further understanding the regulatory control of HT metabolism in plants and its coordination with other biochemical pathways in the metabolic network.
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