ArticleTemperature (Austin, Tex.)
Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay.
Article in Temperature (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 48 citations in OpenAlex.
- Experimenter-free pain assessment in mice using a thermal gradient ring and functional linear models.Pain reports · 2026Article
- Alcohol use disorder-associated pain: clinical and preclinical evidence.Alcohol (Fayetteville, N.Y.) · 2025Review
- WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Pain): methods to assess pain behaviour in rodent models of endometriosis.Molecular human reproduction · 2025Review
- Article
- Automated thermal gradient test for unprovoked assessment of nociceptive preference in rodents.Frontiers in behavioral neuroscience · 2025Article
- Revisiting Thermal Gradient Experiments: Effects of Thermal Heterogeneity on Salamander Behavior.Integrative organismal biology (Oxford, England) · 2025Article
- KCNQ1 is an essential mediator of the sex-dependent perception of moderate cold temperatures.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Specific and Nonuniform Brain States during Cold Perception in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Thermal gradient ring for analysis of temperature-dependent behaviors involving TRP channels in mice.The journal of physiological sciences : JPS · 2024Review
- Cold nociception as a measure of hyperalgesia during spontaneous heroin withdrawal in mice.Pharmacology, biochemistry, and behavior · 2024Article
- Consideration of the importance of measuring thermal discomfort in biomedical research.Trends in molecular medicine · 2023Review
- Spinal cord injury in mice amplifies anxiety: A novel light-heat conflict test exposes increased salience of anxiety over heat.Experimental neurology · 2023Article
- Selection of preferred thermal environment and cold-avoidance responses in rats rely on signals transduced by the dorsal portion of the lateral funiculus of the spinal cord.Temperature (Austin, Tex.) · 2023Article
- Thermal gradient ring reveals thermosensory changes in diabetic peripheral neuropathy in mice.Scientific reports · 2022Article
- Thermal gradient ring reveals different temperature-dependent behaviors in mice lacking thermosensitive TRP channels.The journal of physiological sciences : JPS · 2022Article
- Thermoregulatory heat-escape/cold-seeking behavior in mice and the influence of TRPV1 channels.PloS one · 2022Article
- The HumanFrontiers in molecular neuroscience · 2022Article
- Wide-Range Measurement of Thermal Preference-A Novel Method for Detecting Analgesics Reducing Thermally-Evoked Pain in Mice.Molecules (Basel, Switzerland) · 2021Article
- Exploration of TRPM8 Binding Sites by β-Carboline-Based Antagonists and Their In Vitro Characterization and In Vivo Analgesic Activities.Journal of medicinal chemistry · 2020Article
- A Thermal Place Preference Test for Discovery of Neuropathic Pain Drugs.ACS chemical neuroscience · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have betimes created conflicting results in the literature. Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sensitive enough to detect the contribution of ion channels to cold sensing because in mice smaller alterations in cold nociception do not manifest as frank behavioral changes. To overcome current limitations we have designed a novel device that is automated, provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias. The device represents a thermal gradient assay designed as a circular running track. It allows discerning exploratory behavior from thermal selection behavior and provides increased accuracy by providing measured values in duplicate and by removing edge artifacts. Our custom-designed automated offline analysis by a blob detection algorithm is devoid of movement artifacts, removes light reflection artifacts and provides an internal quality control parameter which we validated. The assay delivers discrete information on a large range of parameters extracted from the occupancy of thermally defined zones such as preference temperature and skew of the distribution. We demonstrate that the assay allows increasingly accurate phenotyping of thermal sensitivity in transgenic mice by disclosing yet unrecognized details on the phenotypes of TRPM8-, TRPA1- and TRPM8/A1-deficient mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.