ArticleThe Journal of biological chemistry2016
Preferential Phosphorylation on Old Histones during Early Mitosis in Human Cells.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Oxidative DNA lesions destabilize centromeres and drive chromosome instability.bioRxiv : the preprint server for biology · 2026Article
- Asymmetric Chromosome Establishment and Segregation During Germline Stem Cell Division.Advances in experimental medicine and biology · 2026Review
- Increased levels of lagging strand polymerase α in an adult stem cell lineage affect replication-coupled histone incorporation.Science advances · 2025Article
- Mitotic chromatin marking governs the segregation of DNA damage.Nature communications · 2025Article
- Roles for the 3D genome in the cell cycle, DNA replication, and double strand break repair.Frontiers in cell and developmental biology · 2025Review
- The CECR2 bromodomain displays distinct binding modes to select for acetylated histone proteins versus non-histone ligands.bioRxiv : the preprint server for biology · 2024Article
- Mitotic chromatin marking governs asymmetric segregation of DNA damage.bioRxiv : the preprint server for biology · 2024Article
- Reduced Levels of Lagging Strand Polymerases Shape Stem Cell Chromatin.bioRxiv : the preprint server for biology · 2024Article
- Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions.EMBO reports · 2023Article
- Studying histone inheritance in different systems using imaging-based methods and perspectives.Biochemical Society transactions · 2023Article
- A single N-terminal amino acid determines the distinct roles of histones H3 and H3.3 in the Drosophila male germline stem cell lineage.PLoS biology · 2023Article
- Characterizing crosstalk in epigenetic signaling to understand disease physiology.The Biochemical journal · 2023Article
- Differential condensation of sister chromatids acts with Cdc6 to ensure asynchronous S-phase entry in Drosophila male germline stem cell lineage.Developmental cell · 2022Article
- Mitotic drive in asymmetric epigenetic inheritance.Biochemical Society transactions · 2022Article
- Characterization of histone inheritance patterns in the Drosophila female germline.EMBO reports · 2021Article
- Combinatorial Histone H3 Modifications Are Dynamically Altered in Distinct Cell Cycle Phases.Journal of the American Society for Mass Spectrometry · 2021Article
- Rebelled epigenome: histone H3S10 phosphorylation and H3S10 kinases in cancer biology and therapy.Clinical epigenetics · 2020Review
- Superresolution imaging of chromatin fibers to visualize epigenetic information on replicative DNA.Nature protocols · 2020Article
- CTCF sites display cell cycle-dependent dynamics in factor binding and nucleosome positioning.Genome research · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
How histone post-translational modifications (PTMs) are inherited through the cell cycle remains poorly understood. Canonical histones are made in the S phase of the cell cycle. Combining mass spectrometry-based technologies and stable isotope labeling by amino acids in cell culture, we question the distribution of multiple histone PTMs on old versus new histones in synchronized human cells. We show that histone PTMs can be grouped into three categories according to their distributions. Most lysine mono-methylation and acetylation PTMs are either symmetrically distributed on old and new histones or are enriched on new histones. In contrast, most di- and tri-methylation PTMs are enriched on old histones, suggesting that the inheritance of different PTMs is regulated distinctly. Intriguingly, old and new histones are distinct in their phosphorylation status during early mitosis in the following three human cell types: HeLa, 293T, and human foreskin fibroblast cells. The mitotic hallmark H3S10ph is predominantly associated with old H3 at early mitosis and becomes symmetric with the progression of mitosis. This same distribution was observed with other mitotic phosphorylation marks, including H3T3/T6ph, H3.1/2S28ph, and H1.4S26ph but not S28/S31ph on the H3 variant H3.3. Although H3S10ph often associates with the neighboring Lys-9 di- or tri-methylations, they are not required for the asymmetric distribution of Ser-10 phosphorylation on the same H3 tail. Inhibition of the kinase Aurora B does not change the distribution despite significant reduction of H3S10ph levels. However, K9me2 abundance on the new H3 is significantly reduced after Aurora B inhibition, suggesting a cross-talk between H3S10ph and H3K9me2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.