ArticleNucleic acids research2016
EZH2 is required for mouse oocyte meiotic maturation by interacting with and stabilizing spindle assembly checkpoint protein BubRI.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Deubiquitinase USP8 regulates the spindle assembly checkpoint in oocytes.Science advances · 2026Article
- A comprehensive review of histone modifications during mammalian oogenesis and early embryo development.Histochemistry and cell biology · 2025Review
- Coordinated regulation of chromatin modifiers reflects organised epigenetic programming in mouse oocytes.Epigenetics & chromatin · 2025Article
- A Microtubule-Associated Protein Functions in Preventing Oocytes from Evading the Spindle Assembly Checkpoint.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Gestational diabetes mellitus causes genome hyper-methylation of oocyte via increased EZH2.Nature communications · 2025Article
- EZH1/2 plays critical roles in oocyte meiosis prophase I in mice.Biological research · 2024Article
- Essential roles of histone lysine methyltransferases EZH2 and EHMT1 in male embryo development of Phenacoccus solenopsis.Communications biology · 2024Article
- Multi-Omics Analysis Reveals Translational Landscapes and Regulations in Mouse and Human Oocyte Aging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Transient Polycomb activity represses developmental genes in growing oocytes.Clinical epigenetics · 2022Article
- A GRIP-1-EZH2 switch binding to GATA-4 is linked to the genesis of rhabdomyosarcoma through miR-29a.Oncogene · 2022Article
- Human sperm TMEM95 binds eggs and facilitates membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Dynamic changes of histone methylation in mammalian oocytes and early embryos.Histochemistry and cell biology · 2022Review
- DZNep promotes mouse bone defect healing via enhancing both osteogenesis and osteoclastogenesis.Stem cell research & therapy · 2021Article
- The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability.International journal of molecular sciences · 2021Review
- The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.Science advances · 2020Article
- TGF-β-activated lncRNA LINC00115 is a critical regulator of glioma stem-like cell tumorigenicity.EMBO reports · 2019Article
- Frequent epigenetic alterations in polycomb repressive complex 2 in osteosarcoma cell lines.Oncotarget · 2018Article
- PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation.Cell cycle (Georgetown, Tex.) · 2018Article
- Molecular determinants of the meiotic arrests in mammalian oocytes at different stages of maturation.Cell cycle (Georgetown, Tex.)Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enhancer of zeste homolog 2 (EZH2) trimethylates histone H3 Lys 27 and plays key roles in a variety of biological processes. Stability of spindle assembly checkpoint protein BubR1 is essential for mitosis in somatic cells and for meiosis in oocytes. However, the role of EZH2 in oocyte meiotic maturation was unknown. Here, we presented a mechanism underlying EZH2 control of BubR1 stability in the meiosis of mouse oocytes. We identified a methyltransferase activity-independent function of EZH2 by demonstrating that EZH2 regulates spindle assembly and the polar body I extrusion. EZH2 was increased with the oocyte progression from GVBD to MII, while EZH2 was concentrated on the chromosomes. Interestingly, inhibition of EZH2 methyltranferase activity by DZNep or GSK343 did not affect oocyte meiotic maturation. However, depletion of EZH2 by morpholino led to chromosome misalignment and abnormal spindle assembly. Furthermore, ectopic expression of EZH2 led to oocyte meiotic maturation arrested at the MI stage followed by chromosome misalignment and aneuploidy. Mechanistically, EZH2 directly interacted with and stabilized BubR1, an effect driving EZH2 into the concert of meiosis regulation. Collectively, we provided a paradigm that EZH2 is required for mouse oocyte meiotic maturation.
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