Evidence map›Paper›PMID 27226347›Full record

ArticleScientific reports2016

The Evx1/Evx1as gene locus regulates anterior-posterior patterning during gastrulation.

Charles C Bell, Paulo P Amaral, Anton Kalsbeek, Graham W Magor, Kevin R Gillinder, Pierre Tangermann, Lorena di Lisio, Seth W Cheetham, Franziska Gruhl, Jessica Frith and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. RNA, Genome Output and Input.Frontiers in genetics · 2020
    Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 1 country.

Charles C BellMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Paulo P AmaralThe Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
Anton KalsbeekThe Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
Graham W MagorMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Kevin R GillinderMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Pierre TangermannThe Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
Lorena di LisioMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Seth W CheethamMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Franziska GruhlMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Jessica FrithThe Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
Michael R TallackMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
Ke-Lin RuThe Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
Joanna CrawfordThe Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
John S MattickGarvan Institute of Medical Research, Sydney, Australia.
Marcel E DingerGarvan Institute of Medical Research, Sydney, Australia.
Andrew C PerkinsMater Research, Translational Research Institute, University of Queensland, Brisbane, Queensland, 4101, Australia.
University of Queensland · AUGarvan Institute of Medical Research · AUTranslational Research Institute · AUMater Research · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thousands of sense-antisense mRNA-lncRNA gene pairs occur in the mammalian genome. While there is usually little doubt about the function of the coding transcript, the function of the lncRNA partner is mostly untested. Here we examine the function of the homeotic Evx1-Evx1as gene locus. Expression is tightly co-regulated in posterior mesoderm of mouse embryos and in embryoid bodies. Expression of both genes is enhanced by BMP4 and WNT3A, and reduced by Activin. We generated a suite of deletions in the locus by CRISPR-Cas9 editing. We show EVX1 is a critical downstream effector of BMP4 and WNT3A with respect to patterning of posterior mesoderm. The lncRNA, Evx1as arises from alternative promoters and is difficult to fully abrogate by gene editing or siRNA approaches. Nevertheless, we were able to generate a large 2.6 kb deletion encompassing the shared promoter with Evx1 and multiple additional exons of Evx1as. This led to an identical dorsal-ventral patterning defect to that generated by micro-deletion in the DNA-binding domain of EVX1. Thus, Evx1as has no function independent of EVX1, and is therefore unlikely to act in trans. We predict many antisense lncRNAs have no specific trans function, possibly only regulating the linked coding genes in cis.

Indexed as

AnimalsBody PatterningBone Morphogenetic Protein 4Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsEmbryo, MammalianGastrulationGene EditingGene Expression Regulation, DevelopmentalHomeodomain ProteinsMiceRNA, Long NoncodingWnt3A ProteinBmp4 protein, mouseBone Morphogenetic Protein 4Evx1 protein, mouseHomeodomain ProteinsRNA, Long NoncodingWnt3A ProteinWnt3a protein, mouse

Identifiers

PMID27226347
PMCPMC4880930
OpenAlexW2402722778

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.