Evidence map›Paper›PMID 27225900›Full record

ArticlePlant physiology2016

Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants.

Na Liu, Ke Xie, Qi Jia, Jinping Zhao, Tianyuan Chen, Huangai Li, Xiang Wei, Xianmin Diao, Yiguo Hong, Yule Liu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed
22.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 119 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Millets for a sustainable future.Journal of experimental botany · 2025
    Review
  6. Article
  7. Review
  8. Article
  9. Virus-Induced Gene Silencing (VIGS) inPlants (Basel, Switzerland) · 2024
    Article
  10. Review
  11. Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024
    Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Na LiuCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Ke XieCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Qi JiaCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Jinping ZhaoCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Tianyuan ChenCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Huangai LiCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Xiang WeiCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Xianmin DiaoCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Yiguo HongCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.).
Yule LiuCenter for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (N.L., K.X., Q.J., J.Z., T.C., X.W., H.L., Y.L.); Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.D.); Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China (Y.H.); and Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China (J.Z.) yuleliu@mail.tsinghua.edu.cn.ORCID 0000-0002-4423-6045
Beijing Normal University · CNHangzhou Normal University · CNTsinghua University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail millet (Setaria italica). This is evidenced by FoMV-based silencing of phytoene desaturase (PDS) and magnesium chelatase in barley, of PDS and Cloroplastos alterados1 in foxtail millet and wheat, and of an additional gene IspH in foxtail millet. Silencing of these genes resulted in photobleached or chlorosis phenotypes in barley, wheat, and foxtail millet. Furthermore, our FoMV-based gene silencing is the first VIGS system reported for foxtail millet, an important C4 model plant. It may provide an efficient toolbox for high-throughput functional genomics in economically important monocot crops.

Indexed as

Gene SilencingGenetic VectorsHordeumOxidoreductasesPlant ProteinsPotexvirusSetaria PlantTriticumOxidoreductasesphytoene dehydrogenasePlant Proteins

Identifiers

PMID27225900
PMCPMC4936545
OpenAlexW2407693019

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.