ArticleScientific reports2016
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Exploring In Vitro Mesenchymal Stem Cell Osteodifferentiation via Vibrational Microspectroscopy: A Review.Stem cell reviews and reports · 2025Review
- Article
- Between Fate Choice and Self-Renewal-Heterogeneity of Adult Neural Crest-Derived Stem Cells.Frontiers in cell and developmental biology · 2021Review
- A specific aggregation-induced emission-conjugated polymer enables visual monitoring of osteogenic differentiation.Bioactive materials · 2020Article
- Transcriptome Analysis Reveals High Similarities between Adult Human Cardiac Stem Cells and Neural Crest-Derived Stem Cells.Biology · 2020Article
- Article
- Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging.Frontiers in bioengineering and biotechnology · 2020Review
- Optical Metric Assessed Engineered Tissues Over a Range of Viability States.Tissue engineering. Part C, Methods · 2019Article
- Live-imaging of Bioengineered Cartilage Tissue using Multimodal Non-linear Molecular Imaging.Scientific reports · 2019Article
- Identification of a Novel High Yielding Source of Multipotent Adult Human Neural Crest-Derived Stem Cells.Stem cell reviews and reports · 2018Article
- Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status.Scientific reports · 2018Article
- Fast epi-detected broadband multiplex CARS and SHG imaging of mouse skull cells.Biomedical optics express · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy were used to detect and image osteogenic differentiation of human neural crest-derived inferior turbinate stem cells (ITSCs). Combined CARS and SHG microscopy was able to detect markers of osteogenesis within 14 days after osteogenic induction. This process increased during continued differentiation. Furthermore, Raman spectroscopy showed significant increases of the PO4(3-) symmetric stretch vibrations at 959 cm(-1) assigned to calcium hydroxyapatite between days 14 and 21. Additionally, CARS microscopy was able to image calcium hydroxyapatite deposits within 14 days following osteogenic induction, which was confirmed by Alizarin Red-Staining and RT- PCR. Taken together, the multimodal label-free analysis methods Raman spectroscopy, CARS and SHG microscopy can monitor osteogenic differentiation of adult human stem cells into osteoblasts with high sensitivity and spatial resolution in three dimensions. Our findings suggest a great potential of these optical detection methods for clinical applications including in vivo observation of bone tissue-implant-interfaces or disease diagnosis.
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