Evidence map›Paper›PMID 27225733›Full record

ArticleScientific reports2016

Human iPSC-derived osteoblasts and osteoclasts together promote bone regeneration in 3D biomaterials.

Ok Hee Jeon, Leelamma M Panicker, Qiaozhi Lu, Jeremy J Chae, Ricardo A Feldman, Jennifer H Elisseeff

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
90citing papers in PubMed, 1 pooled it
10.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

90 citing papers in PubMed, 1 synthesis or guideline pooled it, 153 citations in OpenAlex.

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  7. CFTR mutation is associated with bone differentiation abnormalities in cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2025
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30 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ok Hee JeonTranslational Tissue Engineering Center, Wilmer Eye Institute and the Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Leelamma M PanickerDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Qiaozhi LuTranslational Tissue Engineering Center, Wilmer Eye Institute and the Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA.
Jeremy J ChaeTranslational Tissue Engineering Center, Wilmer Eye Institute and the Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Ricardo A FeldmanDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Jennifer H ElisseeffTranslational Tissue Engineering Center, Wilmer Eye Institute and the Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Johns Hopkins University · USUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone substitutes can be designed to replicate physiological structure and function by creating a microenvironment that supports crosstalk between bone and immune cells found in the native tissue, specifically osteoblasts and osteoclasts. Human induced pluripotent stem cells (hiPSC) represent a powerful tool for bone regeneration because they are a source of patient-specific cells that can differentiate into all specialized cell types residing in bone. We show that osteoblasts and osteoclasts can be differentiated from hiPSC-mesenchymal stem cells and macrophages when co-cultured on hydroxyapatite-coated poly(lactic-co-glycolic acid)/poly(L-lactic acid) (HA-PLGA/PLLA) scaffolds. Both cell types seeded on the PLGA/PLLA especially with 5% w/v HA recapitulated the tissue remodeling process of human bone via coupling signals coordinating osteoblast and osteoclast activity and finely tuned expression of inflammatory molecules, resulting in accelerated in vitro bone formation. Following subcutaneous implantation in rodents, co-cultured hiPSC-MSC/-macrophage on such scaffolds showed mature bone-like tissue formation. These findings suggest the importance of coupling matrix remodeling through osteoblastic matrix deposition and osteoclastic tissue resorption and immunomodulation for tissue development.

Indexed as

Tissue ScaffoldsAnimalsBone RegenerationCell DifferentiationCells, CulturedCoculture TechniquesCulture MediaCytokinesDurapatiteExtracellular MatrixFemaleHeterograftsHumansInduced Pluripotent Stem CellsLactic AcidMacrophagesCulture MediaCytokinesDurapatiteLactic AcidOsteoprotegerinPolyestersPolyglycolic AcidPolylactic Acid-Polyglycolic Acid Copolymerpoly(lactide)RANK LigandTNFRSF11B protein, humanTNFSF11 protein, human

Identifiers

PMID27225733
PMCPMC4881234
OpenAlexW2405633121

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.