ArticleScientific reports2016
CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 105 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
105 citing papers in PubMed, 273 citations in OpenAlex.
- Evaluation of CRISPR/Cas9 based single and multiplex gRNAs against ChiLCV in tobacco.Journal, genetic engineering & biotechnology · 2026Article
- Genome editing‑based strategies to combat geminiviruses: CRISPR/Cas9 and emerging high‑fidelity tools.Archives of microbiology · 2026Review
- Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.BMC plant biology · 2026Article
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- Unveiling the landscape of plant virology in Saudi Arabia: seven decades of progress and future directions toward Vision 2030.Frontiers in plant science · 2026Review
- Review
- Potential for Duplexed, In-Tandem gRNA-Mediated Suppression of Two Essential Genes of Tomato Leaf Curl New Delhi Virus in Crop Plants.Pathogens (Basel, Switzerland) · 2025Article
- A comprehensive atlas of full-length Arabidopsis eccDNA populations identifies their genomic origins and epigenetic regulation.PLoS biology · 2025Article
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- CRISPR-Cas-Based Diagnosis of Geminiviruses.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants.Plants (Basel, Switzerland) · 2024Review
- Pattern-Triggered Immunity and Effector-Triggered Immunity: crosstalk and cooperation of PRR and NLR-mediated plant defense pathways during host-pathogen interactions.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Emerging evidence of seed transmission of begomoviruses: implications in global circulation and disease outbreak.Frontiers in plant science · 2024Review
- Precision Genome Editing Techniques in Gene Therapy: Current State and Future Prospects.Current gene therapy · 2024Review
- A Review of Interactions between Plants and Whitefly-Transmitted Begomoviruses.Plants (Basel, Switzerland) · 2023Review
- Advances and Prospects of Virus-Resistant Breeding in Tomatoes.International journal of molecular sciences · 2023Review
- Article
- Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing.The plant genome · 2023Review
- Comparative Genome Analysis of Old World and New World TYLCV Reveals a Biasness toward Highly Variable Amino Acids in Coat Protein.Plants (Basel, Switzerland) · 2023Article
- Review
45 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 system has recently been used to confer molecular immunity against several eukaryotic viruses, including plant DNA geminiviruses. Here, we provide a detailed analysis of the efficiencies of targeting different coding and non-coding sequences in the genomes of multiple geminiviruses. Moreover, we analyze the ability of geminiviruses to evade the CRISPR/Cas9 machinery. Our results demonstrate that the CRISPR/Cas9 machinery can efficiently target coding and non-coding sequences and interfere with various geminiviruses. Furthermore, targeting the coding sequences of different geminiviruses resulted in the generation of viral variants capable of replication and systemic movement. By contrast, targeting the noncoding intergenic region sequences of geminiviruses resulted in interference, but with inefficient recovery of mutated viral variants, which thus limited the generation of variants capable of replication and movement. Taken together, our results indicate that targeting noncoding, intergenic sequences provides viral interference activity and significantly limits the generation of viral variants capable of replication and systemic infection, which is essential for developing durable resistance strategies for long-term virus control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.