ArticleJournal of clinical microbiology2016
Comparison of Two Widely Used Human Papillomavirus Detection and Genotyping Methods, GP5+/6+-Based PCR Followed by Reverse Line Blot Hybridization and Multiplex Type-Specific E7-Based PCR.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic Test Accuracy of First-Void Urine Human Papillomaviruses for Presence Cervical HPV in Women: Systematic Review and Meta-Analysis.International journal of environmental research and public health · 2021Pooled it
- PCR-LbCas12a Assay for Genotyping of HPV 16 and 18.Molecular biotechnology · 2026Article
- Penile HPV prevalence and associated factors among 20-70-year-old men from Gampaha district in Sri Lanka: a community-based study in a lower middle-income country.BMC infectious diseases · 2026Article
- Human papillomavirus infection and its association with lung cancer: a case-control study (2011-2019).Iranian journal of microbiology · 2025Article
- HPV burden in Armenia among unvaccinated women: a series of cross-sectional population-based prevalence surveys.Vaccine · 2025Article
- HPV Biomarkers in Oral and Blood-Derived Body Fluids in Head and Neck Cancer Patients.Journal of medical virology · 2025Article
- Optimization and analytical validation of the Allplex HPV28 genotyping assay for use in first-void urine samples.Journal of clinical microbiology · 2025Article
- Article
- High burden of human papillomavirus infection among men in Guangzhou, South China: Implications for HPV vaccination strategies.Human vaccines & immunotherapeutics · 2024Observational
- SLC7A11 Expression Is Up-Regulated in HPV- and Tobacco-Associated Lung Cancer.International journal of molecular sciences · 2024Article
- High-risk human papillomavirus distribution according to human immunodeficiency virus status among women with cervical cancer in Abidjan, Côte d'Ivoire, 2018 to 2020.International journal of cancer · 2024Article
- Concentration strategies for spiked and naturally present biomarkers in non-invasively collected first-void urine.European journal of medical research · 2024Article
- Detecting anal human papillomavirus infection in men who have sex with men living with HIV: implications of assay variability.Sexually transmitted infections · 2023Article
- Impact of HPV vaccination on HPV-related oral infections.Oral oncology · 2023Article
- Detection of Circulating HPV16 DNA as a Biomarker for Cervical Cancer by a Bead-Based HPV Genotyping Assay.Microbiology spectrum · 2022Article
- Altered Vaginal Microbiota Composition Correlates With Human Papillomavirus and Mucosal Immune Responses in Women With Symptomatic Cervical Ectopy.Frontiers in cellular and infection microbiology · 2022Article
- Clinical implications of alpha, beta, and gamma HPV infection in juvenile onset recurrent respiratory papillomatosis.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2022Observational
- Prevalence of human papillomavirus types in head and neck cancer sub-sites in the Indian population.Ecancermedicalscience · 2022Article
- Nested PCR followed by NGS: Validation and application for HPV genotyping of Tunisian cervical samples.PloS one · 2021Article
- Impact of Human Papillomavirus Vaccination, Rwanda and Bhutan.Emerging infectious diseases · 2021Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
GP5+/6+-based PCR followed by reverse line blot hybridization (GP5+/6+RLB) and multiplex type-specific PCR (E7-MPG) are two human papillomavirus (HPV) genotyping methodologies widely applied in epidemiological research. We investigated their relative analytical performance in 4,662 samples derived from five studies in Bhutan, Rwanda, and Mongolia coordinated by the International Agency for Research on Cancer (IARC). A total of 630 samples were positive by E7-MPG only (13.5%), 24 were positive by GP5+/6+RLB only (0.5%), and 1,014 were positive (21.8%) by both methods. Ratios of HPV type-specific positivity of the two tests (E7-MPG:GP5+/6+RLB ratio) were calculated among 1,668 samples that were HPV positive by one or both tests. E7-MPG:GP5+/6+RLB ratios were >1 for all types and highly reproducible across populations and sample types. E7-MPG:GP5+/6+RLB ratios were highest for HPV53 (7.5) and HPV68 (7.1). HPV16 (1.6) and HPV18 (1.7) had lower than average E7-MPG:GP5+/6+RLB ratios. Among E7-MPG positive infections, median mean fluorescence intensity (MFI; a semiquantitative measure of viral load) tended to be higher among samples positive for the same virus type by GP5+/6+RLB than for those negative for the same type by GP5+/6+RLB. Exceptions, however, included HPV53, -59, and -82, for which the chances of being undetected by GP5+/6+RLB appeared to be MFI independent. Furthermore, the probability of detecting an additional type by E7-MPG was higher when another type was already detected by GP5+/6+RLB, suggesting the existence of masking effects due to competition for GP5+/6+ PCR primers. In conclusion, this analysis is not an evaluation of clinical performance but may inform choices for HPV genotyping methods in epidemiological studies, when the relative merits and dangers of sensitivity versus specificity for individual types should be considered, as well as the potential to unmask nonvaccine types following HPV vaccination.
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