ArticleBiophysical journal2016
Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Membrane fusion studied by colloidal probes.European biophysics journal : EBJ · 2021Review
- Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs.Scientific reports · 2017Article
- SNARE-mediated membrane fusion trajectories derived from force-clamp experiments.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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Authors and funding
8 authors.
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Abstract
The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.e., an optical fusion assay based on membrane-coated glass spheres. We present a technique to analyze membrane-membrane interactions in a large ensemble of particles. Detailed information on the geometry of the fusion stalk of fully fused membranes is obtained by studying the diffusional lipid dynamics with fluorescence recovery after photobleaching experiments. A small contact zone is a strong obstruction for the particle exchange across the fusion spot. With the aid of computer simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-membrane-coated beads allow us to estimate the size of the contact zones between two membrane-coated beads. Minimizing delamination and bending energy leads to minimal angles close to those geometrically allowed.
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