ArticleeLife2016
A bend, flip and trap mechanism for transposon integration.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Information storage across a microbial community using universal RNA barcoding.Nature biotechnology · 2026Article
- Emerging trends in genome integration tools for precision engineering of diverse bacterial species.Synthetic biology (Oxford, England) · 2025Review
- Article
- Article
- Structural insight into Tn3 family transposition mechanism.Nature communications · 2022Article
- Engineered Sleeping Beauty transposase redirects transposon integration away from genes.Nucleic acids research · 2022Article
- Structure and function of retroviral integrase.Nature reviews. Microbiology · 2022Review
- Jumping Ahead withViruses · 2021Review
- Structural basis for the activation and suppression of transposition during evolution of the RAG recombinase.The EMBO journal · 2020Article
- Structural basis of seamless excision and specific targeting by piggyBac transposase.Nature communications · 2020Article
- How mouse RAG recombinase avoids DNA transposition.Nature structural & molecular biology · 2020Article
- Jump ahead with a twist: DNA acrobatics drive transposition forward.Current opinion in structural biology · 2019Review
- Article
- Structure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.Nature structural & molecular biology · 2019Article
- Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.Nucleic acids research · 2018Article
- Article
- A single active site in the mariner transposase cleaves DNA strands of opposite polarity.Nucleic acids research · 2017Article
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Target DNA bending by the Mu transpososome promotes careful transposition and prevents its reversal.eLife · 2017Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with transposon ends covalently joined to target DNA, portrays the transposition machinery after DNA integration. It reveals severe distortion of target DNA and flipping of the target adenines into extra-helical positions. Fluorescence experiments confirm dynamic base flipping in solution. Transposase residues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient transposon integration and transposition in vitro. Transposase recognises the flipped target adenines via base-specific interactions with backbone atoms, offering a molecular basis for TA target sequence selection. Our results will provide a template for re-designing mariner/Tc1 transposases with modified target specificities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.