ArticleCancers2016
The MYC 3' Wnt-Responsive Element Drives Oncogenic MYC Expression in Human Colorectal Cancer Cells.
Article in Cancers, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma.BioMed research international · 2020Trial
- TCF7L1 regulates colorectal cancer cell migration by repressing GAS1 expression.Scientific reports · 2024Article
- TCF7L1 RegulatesGenes · 2023Article
- Transcriptome Analyses Identify DeregulatedBiomolecules · 2022Article
- TCF 4 tumor suppressor: a molecular target in the prognosis of sporadic colorectal cancer in humans.Cellular & molecular biology letters · 2020Article
- Tristetraprolin targets Nos2 expression in the colonic epithelium.Scientific reports · 2019Article
- The Crohn's disease associated SNP rs6651252 impacts MYC gene expression in human colonic epithelial cells.PloS one · 2019Article
- Effects of a single transient transfection of Ten-eleven translocation 1 catalytic domain on hepatocellular carcinoma.PloS one · 2018Article
- RNA-seq implicates deregulation of the immune system in the pathogenesis of diverticulitis.American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Colorectal cancer and theGenomics data · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mutations in components of the Wnt/β-catenin signaling pathway drive colorectal cancer (CRC) by deregulating expression of downstream target genes including the c-MYC proto-oncogene (MYC). The critical regulatory DNA enhancer elements that control oncogenic MYC expression in CRC have yet to be fully elucidated. In previous reports, we correlated T-cell factor (TCF) and β-catenin binding to the MYC 3' Wnt responsive DNA element (MYC 3' WRE) with MYC expression in HCT116 cells. Here we used CRISPR/Cas9 to determine whether this element is a critical driver of MYC. We isolated a clonal population of cells that contained a deletion of a single TCF binding element (TBE) within the MYC 3' WRE. This deletion reduced TCF/β-catenin binding to this regulatory element and decreased MYC expression. Using RNA-Seq analysis, we found altered expression of genes that regulate metabolic processes, many of which are known MYC target genes. We found that 3' WRE-Mut cells displayed a reduced proliferative capacity, diminished clonogenic growth, and a decreased potential to form tumors in vivo. These findings indicate that the MYC 3' WRE is a critical driver of oncogenic MYC expression and suggest that this element may serve as a therapeutic target for CRC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.