ArticleProceedings of the National Academy of Sciences of the United States of America2016
Molecular tandem repeat strategy for elucidating mechanical properties of high-strength proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 90 citations in OpenAlex.
- Technologies for engineering repetitive DNA.Quantitative biology (Beijing, China) · 2026Review
- Synthesis of Ultra-Large Fibrous Proteins from Bacteria via a Looped-Translation System.bioRxiv : the preprint server for biology · 2026Article
- High-throughput screening of FRET-based proteins using a hyperspectral microcapillary array.iScience · 2026Article
- Cephalopod proteins for bioinspired and sustainable biomaterials design.Materials today. Bio · 2025Review
- A Novel Gene Synthesis Platform for Designing Functional Protein Polymers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Order-Disorder Balance in Silk-Elastin-like Polypeptides Determines Their Self-Assembly into Hydrogel Networks.ACS applied materials & interfaces · 2025Article
- A PDZ tandem repeat folds and unfolds via different pathways.Protein science : a publication of the Protein Society · 2024Article
- Article
- Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers.International journal of molecular sciences · 2023Review
- Protein-Based Biological Materials: Molecular Design and Artificial Production.Chemical reviews · 2023Review
- Bioinspired stretchable molecular composites of 2D-layered materials and tandem repeat proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Characterization of gliadin, secalin and hordein fractions using analytical techniques.Scientific reports · 2021Article
- Article
- Optimized Loopable Translation as a Platform for the Synthesis of Repetitive Proteins.ACS central science · 2021Article
- Microbial production of megadalton titin yields fibers with advantageous mechanical properties.Nature communications · 2021Article
- Biosynthetic self-healing materials for soft machines.Nature materials · 2020Article
- Multifunctional and biodegradable self-propelled protein motors.Nature communications · 2019Article
- Synthetic biology for fibres, adhesives and active camouflage materials in protection and aerospace.MRS communications · 2019Article
- Squid-Inspired Tandem Repeat Proteins: Functional Fibers and Films.Frontiers in chemistry · 2019Review
- Silk fibroin hydrogels from the Colombian silkworm Bombyx mori L: Evaluation of physicochemical properties.PloS one · 2019Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many globular and structural proteins have repetitions in their sequences or structures. However, a clear relationship between these repeats and their contribution to the mechanical properties remains elusive. We propose a new approach for the design and production of synthetic polypeptides that comprise one or more tandem copies of a single unit with distinct amorphous and ordered regions. Our designed sequences are based on a structural protein produced in squid suction cups that has a segmented copolymer structure with amorphous and crystalline domains. We produced segmented polypeptides with varying repeat number, while keeping the lengths and compositions of the amorphous and crystalline regions fixed. We showed that mechanical properties of these synthetic proteins could be tuned by modulating their molecular weights. Specifically, the toughness and extensibility of synthetic polypeptides increase as a function of the number of tandem repeats. This result suggests that the repetitions in native squid proteins could have a genetic advantage for increased toughness and flexibility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.