ArticleInternational journal of molecular medicine2016
Magnetically induced electrostimulation of human osteoblasts results in enhanced cell viability and osteogenic differentiation.
Article in International journal of molecular medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- A validated in vitro approach for low-frequency alternating-current stimulation of stem cells: From electrode characterization to biological responses.Protoplasma · 2026Article
- Parameter-resolved AC electrical stimulation links electrical system characteristics to early transcriptional responses in human stem cells.Frontiers in bioengineering and biotechnology · 2026Article
- Accelerated Bone Healing via Electrical Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Thermodynamic Considerations on the Biophysical Interaction between Low-Energy Electromagnetic Fields and Biosystems.Membranes · 2024Article
- Effects of electrical stimulation with alternating fields on the osseointegration of titanium implants in the rabbit tibia - a pilot study.Frontiers in bioengineering and biotechnology · 2024Article
- Signalling pathways underlying pulsed electromagnetic fields in bone repair.Frontiers in bioengineering and biotechnology · 2024Review
- Cell-cell interactions and fluctuations in the direction of motility promote directed migration of osteoblasts in direct current electrotaxis.Frontiers in bioengineering and biotechnology · 2022Article
- Role of KInternational journal of molecular sciences · 2021Review
- Anodic TiONanomaterials (Basel, Switzerland) · 2021Review
- The Antimicrobial and Anti-Inflammatory Effects of Silver Nanoparticles Synthesised fromNanomaterials (Basel, Switzerland) · 2021Article
- Establishment of a New Device for Electrical Stimulation of Non-Degenerative Cartilage Cells In Vitro.International journal of molecular sciences · 2021Article
- Alternating Electric Fields Modify the Function of Human Osteoblasts Growing on and in the Surroundings of Titanium Electrodes.International journal of molecular sciences · 2020Article
- Establishment and Evaluation of an In Vitro System for Biophysical Stimulation of Human Osteoblasts.Cells · 2020Article
- Bioelectronics-on-a-chip for cardio myoblast proliferation enhancement using electric field stimulation.Biomaterials research · 2020Article
- Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields.Journal of clinical medicine · 2019Article
- Stimulation of Human Osteoblast Differentiation in Magneto-Mechanically Actuated Ferromagnetic Fiber Networks.Journal of clinical medicine · 2019Article
- Human Osteoblast Migration in DC Electrical Fields Depends on Store Operated CaFrontiers in bioengineering and biotechnology · 2019Article
- Effect of electric stimulation on human chondrocytes and mesenchymal stem cells under normoxia and hypoxia.Molecular medicine reports · 2018Article
- Alendronate promotes osteoblast differentiation and bone formation in ovariectomy-induced osteoporosis through interferon-β/signal transducer and activator of transcription 1 pathway.Experimental and therapeutic medicine · 2018Article
- Extremely low frequency pulsed electromagnetic fields cause antioxidative defense mechanisms in human osteoblasts via induction of •OScientific reports · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of electromagnetic fields to support the bone-healing processes is a therapeutic approach for patients with musculoskeletal disorders. The ASNIS-III s-series screw is a bone stimulation system providing electromagnetic stimulation; however, its influence on human osteoblasts (hOBs) has not been extensively investigated. Therefore, in the present study, the impact of this system on the viability and differentiation of hOBs was examined. We used the ASNIS-III s screw system in terms of a specific experimental test set-up. The ASNIS-III s screw system was used for the application of electromagnetic fields (EMF, 3 mT, 20 Hz) and electromagnetic fields combined with an additional alternating electric field (EMF + EF) (3 mT, 20 Hz, 700 mV). The stimulation of primary hOBs was conducted 3 times per day for 45 min over a period of 72 h. Unstimulated cells served as the controls. Subsequently, the viability, the gene expression of differentiation markers and pro-collagen type 1 synthesis of the stimulated osteoblasts and corresponding controls were investigated. The application of both EMF and EMF + EF using the ASNIS-III s screw system revealed a positive influence on bone cell viability and moderately increased the synthesis of pro-collagen type 1 compared to the unstimulated controls. Stimulation with EMF resulted in a slightly enhanced gene expression of type 1 collagen and osteocalcin; however, stimulation with EMF + EF resulted in a significant increase in alkaline phosphatase (1.4-fold) and osteocalcin (1.6-fold) levels, and a notable increase in the levels of runt-related transcription factor 2 (RUNX-2; 1.54-fold). Our findings demonstrate that stimulation with electromagnetic fields and an additional alternating electric field has a positive influence on hOBs as regards cell viability and the expression of osteoblastic differentiation markers.
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