ArticleCellular microbiology2016
Ultrastructural characterization of membranous torovirus replication factories.
Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Conserved structural features of RNA export pores spanning the double membrane of arterivirus and coronavirus replication organelles.Journal of virology · 2026Article
- The DMV pore-forming TM2-Y region of SARS-CoV-2 nsp3 exhibits structural conservation beyond the coronavirus family.Journal of virology · 2026Article
- Mechanism, structural and functional insights into nidovirus-induced double-membrane vesicles.Frontiers in immunology · 2024Review
- Reverse Genetics with a Full-Length Infectious cDNA Clone of Bovine Torovirus.Journal of virology · 2022Article
- Article
- Genome-scale CRISPR screen identifies TMEM41B as a multi-function host factor required for coronavirus replication.PLoS pathogens · 2021Article
- Recent Progress in Torovirus Molecular Biology.Viruses · 2021Review
- The Interplay Between Coronavirus and Type I IFN Response.Frontiers in microbiology · 2021Review
- Exploiting Connections for Viral Replication.Frontiers in cell and developmental biology · 2021Article
- Double-Membrane Vesicles as Platforms for Viral Replication.Trends in microbiology · 2020Review
- Porcine Epidemic Diarrhea Virus and the Host Innate Immune Response.Pathogens (Basel, Switzerland) · 2020Review
- Article
- Activation of the autophagy pathway by Torovirus infection is irrelevant for virus replication.PloS one · 2019Article
- Article
- Antiviral activity of K22 against members of the order Nidovirales.Virus research · 2018Article
- Article
- Negri bodies are viral factories with properties of liquid organelles.Nature communications · 2017Article
- Ultrastructural characterization of membranous torovirus replication factories.Cellular microbiology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plus-stranded RNA viruses replicate in the cytosol of infected cells, in membrane-bound replication complexes containing the replicase proteins, the viral RNA and host proteins. The formation of the replication and transcription complexes (RTCs) through the rearrangement of cellular membranes is currently being actively studied for viruses belonging to different viral families. In this work, we identified double-membrane vesicles (DMVs) in the cytoplasm of cells infected with the equine torovirus Berne virus (BEV), the prototype member of the Torovirus genus (Coronaviridae family, Nidovirales order). Using confocal microscopy and transmission electron microscopy, we observed a close relationship between the RTCs and the DMVs of BEV. The examination of BEV-infected cells revealed that the replicase proteins colocalize with each other and with newly synthesized RNA and are associated to the membrane rearrangement induced by BEV. However, the double-stranded RNA, an intermediate of viral replication, is exclusively limited to the interior of DMVs. Our results with BEV resemble those obtained with other related viruses in the Nidovirales order, thus providing new evidence to support the idea that nidoviruses share a common replicative structure based on the DMV arranged clusters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.