ArticlePlant physiology2016
A Laser Dissection-RNAseq Analysis Highlights the Activation of Cytokinin Pathways by Nod Factors in the Medicago truncatula Root Epidermis.
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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68citing papers in PubMed
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68 citing papers in PubMed, 153 citations in OpenAlex.
- A laser capture microdissection-based transcriptome analysis on salt glands highlights salt secretion mechanism in mangrove plant Avicennia marina.The Plant journal : for cell and molecular biology · 2026Article
- A recently evolved Nodulin recruited by NIN for root hair rhizobial infection in Medicago truncatula.The New phytologist · 2026Article
- Symbiosis-associated UMAMIT transporters required for establishing efficient nitrogen fixation in Medicago truncatula.The New phytologist · 2026Article
- Rhizobial Nod factors modulate reactive oxygen species, jasmonates, and pattern-recognizing receptors to suppress immune response.Plant molecular biology · 2026Article
- A legume cellulase required for rhizobial infection and colonization in root nodule symbiosis.Nature communications · 2025Article
- Investigating biological nitrogen fixation via single-cell transcriptomics.Journal of experimental botany · 2025Review
- Gibberellin dynamics governing nodulation revealed using GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula lateral organs.The Plant cell · 2024Article
- CEP hormones at the nexus of nutrient acquisition and allocation, root development, and plant-microbe interactions.Journal of experimental botany · 2024Review
- Laser Capture Microdissection Transcriptome Reveals Spatiotemporal Tissue Gene Expression Patterns ofMolecular plant-microbe interactions : MPMI · 2023Article
- Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus.Nature communications · 2023Article
- Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago.Nature plants · 2023Article
- Comparative phylotranscriptomics reveals ancestral and derived root nodule symbiosis programmes.Nature plants · 2023Article
- Plant latent defense response against compatibility.The ISME journal · 2023Review
- ACurrent issues in molecular biology · 2023Article
- The Medicago SymCEP7 hormone increases nodule number via shoots without compromising lateral root number.Plant physiology · 2023Article
- Dancing to a different tune, can we switch from chemical to biological nitrogen fixation for sustainable food security?PLoS biology · 2023Article
- Exogenously Applied Cytokinin Altered the Bacterial Release and Subsequent Stages of Nodule Development in PeaPlants (Basel, Switzerland) · 2023Article
- The B-type response regulator GmRR11d mediates systemic inhibition of symbiotic nodulation.Nature communications · 2022Article
- Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover (Life (Basel, Switzerland) · 2022Article
- Right time, right place: The dynamic role of hormones in rhizobial infection and nodulation of legumes.Plant communications · 2022Review
8 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 3 institutions in 3 countries.
Marie-Françoise JardinaudLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
Stéphane BoivinLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0002-4084-2312
Nathalie RoddeLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0003-3361-4730
Olivier CatriceLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0002-5820-1660
Anna KisialaLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0002-4918-7836
Agnes LepageLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
Sandra MoreauLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
Brice RouxLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0001-6923-0902
Ludovic CottretLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
Erika SalletLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0001-6062-5043
Mathias BraultLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
R J Neil EmeryLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).ORCID 0000-0003-1304-6430
Jérôme GouzyLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
Florian FrugierLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.).
Pascal GamasLIPM, Université de Toulouse, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet-Tolosan, France (M.-F.J., N.R., O.C., A.L., S.M., B.R., L.C., E.S., J.G., P.G.);INPT-Université de Toulouse, ENSAT, 31326 Castanet-Tolosan, France (M.-F.J.);Institute of Plant Sciences-Paris Saclay University, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Universités Paris-Sud/Paris-Diderot/d'Evry, 91190 Gif-sur-Yvette, France (S.B., M.B., F.F.);Biology Department, Trent University, Peterborough, Ontario, Canada K9J 7B8 (A.K., R.J.N.E.); andDepartment of Plant Genetics, Physiology, and Biotechnology, University of Technology and Life Sciences, 85-789 Bydgoszcz, Poland (A.K.) pascal.gamas@toulouse.inra.fr.ORCID 0000-0002-6253-4249
Centre National de la Recherche Scientifique · FRUniversité Paris Cité · FRBydgoszcz University of Science and Technology · PL
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nod factors (NFs) are lipochitooligosaccharidic signal molecules produced by rhizobia, which play a key role in the rhizobium-legume symbiotic interaction. In this study, we analyzed the gene expression reprogramming induced by purified NF (4 and 24 h of treatment) in the root epidermis of the model legume Medicago truncatula Tissue-specific transcriptome analysis was achieved by laser-capture microdissection coupled to high-depth RNA sequencing. The expression of 17,191 genes was detected in the epidermis, among which 1,070 were found to be regulated by NF addition, including previously characterized NF-induced marker genes. Many genes exhibited strong levels of transcriptional activation, sometimes only transiently at 4 h, indicating highly dynamic regulation. Expression reprogramming affected a variety of cellular processes, including perception, signaling, regulation of gene expression, as well as cell wall, cytoskeleton, transport, metabolism, and defense, with numerous NF-induced genes never identified before. Strikingly, early epidermal activation of cytokinin (CK) pathways was indicated, based on the induction of CK metabolic and signaling genes, including the CRE1 receptor essential to promote nodulation. These transcriptional activations were independently validated using promoter:β-glucuronidase fusions with the MtCRE1 CK receptor gene and a CK response reporter (TWO COMPONENT SIGNALING SENSOR NEW). A CK pretreatment reduced the NF induction of the EARLY NODULIN11 (ENOD11) symbiotic marker, while a CK-degrading enzyme (CYTOKININ OXIDASE/DEHYDROGENASE3) ectopically expressed in the root epidermis led to increased NF induction of ENOD11 and nodulation. Therefore, CK may play both positive and negative roles in M. truncatula nodulation.
Indexed as
CytokininsGene Expression Regulation, PlantLasersLipopolysaccharidesMedicago truncatulaPlant EpidermisPlant ProteinsPlant RootsPlants, Genetically ModifiedRoot Nodules, PlantSequence Analysis, RNASignal TransductionCytokininsENOD11 protein, Medicago truncatulaLipopolysaccharidesPlant Proteins
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