Evidence map›Paper›PMID 27217494›Full record

ArticlePlant physiology2016

The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2.

Christopher K Dugard, Rachel A Mertz, Catherine Rayon, Davide Mercadante, Christopher Hart, Matheus R Benatti, Anna T Olek, Phillip J SanMiguel, Bruce R Cooper, Wolf-Dieter Reiter and 2 more

Open access · bronzeAbstract read
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 4 countries.

Christopher K DugardDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).
Rachel A MertzDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0002-2617-3220
Catherine RayonDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0002-0877-9675
Davide MercadanteDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0001-6792-7706
Christopher HartDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0003-0726-5071
Matheus R BenattiDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0001-7179-9153
Anna T OlekDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).
Phillip J SanMiguelDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0002-3742-9527
Bruce R CooperDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).
Wolf-Dieter ReiterDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0003-4704-9453
Maureen C McCannDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.).ORCID 0000-0001-6956-4216
Nicholas C CarpitaDepartment of Botany and Plant Pathology (C.K.D., R.A.M., A.T.O., N.C.C.), Department of Biological Sciences (M.R.B., M.C.M., N.C.C.), Bindley Bioscience Center (B.R.C., M.C.M., N.C.C.), and Department of Horticulture and Landscape Architecture (P.J.S.), Purdue University, West Lafayette, Indiana 47907-2054;Université de Picardie Jules Verne, EA 3900-BIOPI, 80039 Amiens, France (C.R.);Heidelberg Institut für Theoretische Studien, Molecular Biomechanics, 69118 Heidelberg, Germany (D.M.); andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (C.H., W.-D.R.) carpita@purdue.edu.ORCID 0000-0003-0770-314X
Purdue University West Lafayette · USUniversity of Connecticut · USBiologie des Plantes et Innovation · FRI.R.C.C.S. Oasi Maria SS · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional marker-based mapping and next-generation sequencing was used to determine that the Arabidopsis (Arabidopsis thaliana) low cell wall arabinose mutant murus5 (mur5) encodes a defective allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 (RGP2). Marker analysis of 13 F2 confirmed mutant progeny from a recombinant mapping population gave a rough map position on the upper arm of chromosome 5, and deep sequencing of DNA from these 13 lines gave five candidate genes with G→A (C→T) transitions predicted to result in amino acid changes. Of these five, only insertional mutant alleles of RGP2, a gene that encodes a UDP-arabinose mutase that interconverts UDP-arabinopyranose and UDP-arabinofuranose, exhibited the low cell wall arabinose phenotype. The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and overexpression of wild-type RGP2 complementary DNA placed under the control of the 35S promoter in the three alleles. The mur5 mutation results in the conversion of cysteine-257 to tyrosine-257 within a conserved hydrophobic cluster predicted to be distal to the active site and essential for protein stability and possible heterodimerization with other isoforms of RGP.

Indexed as

AllelesArabidopsisArabidopsis ProteinsArabinoseCell WallChromosome MappingChromosomes, PlantGene Expression Regulation, PlantGenetic Complementation TestGlucosyltransferasesHigh-Throughput Nucleotide SequencingMutationPlants, Genetically ModifiedProtein DomainsProtein FoldingProtein StabilityArabidopsis ProteinsArabinoseGlucosyltransferasesRGP2 protein, Arabidopsis

Identifiers

PMID27217494
PMCPMC4936543
OpenAlexW2403697096

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