ArticleScientific reports2016
Integrated Microfluidic Isolation of Aptamers Using Electrophoretic Oligonucleotide Manipulation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Aptamer-based technologies for extracellular vesicle analysis and engineering: from isolation to functional applications.Extracellular vesicles and circulating nucleic acids · 2026Review
- Designing the Future of Biosensing: Advances in Aptamer Discovery, Computational Modeling, and Diagnostic Applications.Biosensors · 2025Review
- Advancements in SELEX Technology for Aptamers and Emerging Applications in Therapeutics and Drug Delivery.Biomolecules · 2025Review
- High-affinity one-step aptamer selection using a non-fouling porous hydrogel.Nature biotechnology · 2024Article
- Assessing Aptamer-Analyte Binding Kinetics by Microfluidic Fluorescence Microscopy.Sensors and actuators. B, Chemical · 2024Article
- A Microfluidic Dual-Aptamer Sandwich Assay for Rapid and Cost-Effective Detection of Recombinant Proteins.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2023Article
- A comparative study of aptamer isolation by conventional and microfluidic strategies.The Analyst · 2023Article
- Aptamer-based Emerging Tools for Viral Biomarker Detection: A Focus on SARS-CoV-2.Current medicinal chemistry · 2023Review
- Microfluidic isolation of aptamers with affinity towards multiple myeloma monoclonal immunoglobulins.Biomedical microdevices · 2022Article
- On-chip selection of adenosine aptamer using graphene oxide-coated magnetic nanoparticles.Biomicrofluidics · 2022Article
- Advances and Challenges in Small-Molecule DNA Aptamer Isolation, Characterization, and Sensor Development.Angewandte Chemie (International ed. in English) · 2021Review
- The Application of Microfluidic Technologies in Aptamer Selection.Frontiers in cell and developmental biology · 2021Review
- Aptamers against Immunoglobulins: Design, Selection and Bioanalytical Applications.International journal of molecular sciences · 2020Review
- Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates.ACS applied materials & interfaces · 2018Article
- Microfluidic methods for aptamer selection and characterization.The Analyst · 2017Review
- Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM.Scientific reports · 2017Article
- An Integrated Microfluidic SELEX Approach Using Combined Electrokinetic and Hydrodynamic Manipulation.SLAS technology · 2017Article
- INTEGRATED MICROFLUIDIC SELEX USING FREE SOLUTION ELECTROKINETICS.Journal of the Electrochemical Society · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We present a microfluidic approach to integrated isolation of DNA aptamers via systematic evolution of ligands by exponential enrichment (SELEX). The approach employs a microbead-based protocol for the processes of affinity selection and amplification of target-binding oligonucleotides, and an electrophoretic DNA manipulation scheme for the coupling of these processes, which are required to occur in different buffers. This achieves the full microfluidic integration of SELEX, thereby enabling highly efficient isolation of aptamers in drastically reduced times and with minimized consumption of biological material. The approach as such also offers broad target applicability by allowing selection of aptamers with respect to targets that are either surface-immobilized or solution-borne, potentially allowing aptamers to be developed as readily available affinity reagents for a wide range of targets. We demonstrate the utility of this approach on two different procedures, respectively for isolating aptamers against a surface-immobilized protein (immunoglobulin E) and a solution-phase small molecule (bisboronic acid in the presence of glucose). In both cases aptamer candidates were isolated in three rounds of SELEX within a total process time of approximately 10 hours.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.