ArticleJournal of neuroscience methods2016
Co-culture model consisting of human brain microvascular endothelial and peripheral blood mononuclear cells.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
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- Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling.Microbiology spectrum · 2025Article
- Co-Culture Models: Key Players in In Vitro Neurotoxicity, Neurodegeneration and BBB Modeling Studies.Biomedicines · 2024Review
- Keeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity.Advanced healthcare materials · 2023Review
- Chronic Low Dose Morphine Does Not Alter Two In Vitro BBB Models.Brain sciences · 2022Article
- Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Blood-Brain Barrier Permeability: Is 5-Hydroxytryptamine Receptor Type 4 a Game Changer?Pharmaceutics · 2021Article
- Review of Design Considerations for Brain-on-a-Chip Models.Micromachines · 2021Review
- Bacteria elevate extracellular adenosine to exploit host signaling for blood-brain barrier disruption.Virulence · 2020Article
- Comparison of the rate of dedifferentiation with increasing passages among cell sources for an in vitro model of the blood-brain barrier.Journal of neural transmission (Vienna, Austria : 1996) · 2020Article
- p95HER2-T cell bispecific antibody for breast cancer treatment.Science translational medicine · 2018Article
- Establishment of a Human iPSC- and Nanofiber-Based Microphysiological Blood-Brain Barrier System.ACS applied materials & interfaces · 2018Article
- New experimental models of the blood-brain barrier for CNS drug discovery.Expert opinion on drug discovery · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundNumerous systems exist to model the blood-brain barrier (BBB) with the goal of understanding the regulation of passage into the central nervous system (CNS) and the potential impact of selected insults on BBB function. These models typically focus on the intrinsic cellular properties of the BBB, yet studies of peripheral cell migration are often excluded due to technical restraints. NEW
methodThis method allows for the study of in vitro cellular transmigration following exposure to any treatment of interest through optimization of co-culture conditions for the human brain microvascular endothelial cells (BMEC) cell line, hCMEC/D3, and primary human peripheral blood mononuclear cells (PBMCs).
resultshCMEC/D3 cells form functionally confluent monolayers on collagen coated polytetrafluoroethylene (PTFE) transwell inserts, as assessed by microscopy and tracer molecule (FITC-dextran (FITC-D)) exclusion. Two components of complete hCMEC/D3 media, EBM-2 base-media and hydrocortisone (HC), were determined to be cytotoxic to PBMCs. By combining the remaining components of complete hCMEC/D3 media with complete PBMC media a resulting co-culture media was established for use in hCMEC/D3-PBMC co-culture functional assays. COMPARISON WITH EXISTING
methodsThrough this method, issues of extensive differences in culture media conditions are resolved allowing for treatments and functional assays to be conducted on the two cell populations co-cultured simultaneously.
conclusionDescribed here is an in vitro co-culture model of the BBB, consisting of the hCMEC/D3 cell line and primary human PBMCs. The co-culture media will now allow for the study of exposure to potential insults to BBB function over prolonged time courses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.