Evidence map›Paper›PMID 27216534›Full record

ArticleMolecular biology reports2016

The c.-190 C>A transversion in promoter region of protamine1 gene as a genetic risk factor for idiopathic oligozoospermia.

Shirin Jamali, Mohammad Karimian, Hossein Nikzad, Younes Aftabi

Abstract read
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In one paragraph

Article in Molecular biology reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 3 pooled it
8.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 3 syntheses or guidelines pooled it, 27 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. IL-6-174 G/C transversion might decrease male infertility risk: A case-control study.International journal of reproductive biomedicine · 2024
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  13. Association of GSTO1 A140D and GSTO2 N142D Gene Variations with Breast Cancer RiskAsian Pacific journal of cancer prevention : APJCP · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Shirin JamaliGenetic Division, Department of Biology, School of Sciences, Islamic Azad University of Ashkezar, Yazd, Iran.
Mohammad KarimianGametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Hossein NikzadGametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran. hnikzad10@gmail.com.
Younes AftabiDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
University of Mazandaran · IRIslamic Azad University, Yazd · IRKashan University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genome condensation in the sperm head is resulted with replacing of histones by protamines during spermatogenesis. It is reported that defects in the protamine 1 (PRM1) and/or 2 (PRM2) genes cause male infertility. Located on chromosome 16 (16p13.2) these genes contain numerous unstudied single nucleotide polymorphisms. This study aimed to investigate the association of c.-190 C>A and g.298 G>C transversions that respectively occur in PRM1 and PRM2 genes with idiopathic oligozoospermia. In a case-control study, we collected blood samples from 130 idiopathic oligozoospermia and 130 fertile men. Detection of c.-190 C>A and g.298 G>C polymorphisms performed by direct sequencing and PCR-RFLP methods respectively. An in silico analysis was performed by ASSP, NetGene 2, and PNImodeler online web servers. Our data revealed that g.298 G>C transversion in PRM2 was not associated with oligozoospermia (P > 0.05). Whereas, -190CA and -190AA genotypes in PRM1 gene were associated significantly with increased risk of oligozoospermia (P = 0.0017 and 0.0103, respectively). Also carriers of A allele (CA+AA) for PRM1 c.-190 C>A were at a high risk for oligozoospermia (OR 3.2440, 95 % CI 1.8060-5.8270, P = 0.0001). Further, in silico analysis revealed that c.-190 C>A transversion may alter transcription factor interactions with the promoter region of PRM1. The results revealed that the c.-190 C>A transversion may involve in the susceptibility for oligozoospermia and could be represented as a noninvasive molecular marker for genetic diagnosis of idiopathic oligozoospermia.

Indexed as

AdultCase-Control StudiesGene FrequencyGenetic Association StudiesGenetic Predisposition to DiseaseHumansMaleOligospermiaPolymorphism, Single NucleotidePromoter Regions, GeneticProtaminesRisk FactorsSequence Analysis, DNAPRM1 protein, humanProtaminesIdiopathic oligozoospermiaIn silico analysisMale infertilityProtamine genesSingle nucleotide polymorphism

Identifiers

PMID27216534
OpenAlexW2398139170

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.