ArticleeLife2016
Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Absolute voltage mapping using dynamic photocycle control.bioRxiv : the preprint server for biology · 2026Article
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids.Journal of molecular and cellular cardiology · 2025Article
- Dendritic excitations govern back-propagation via a spike-rate accelerometer.bioRxiv : the preprint server for biology · 2024Article
- Making Sense of Psychedelics in the CNS.The international journal of neuropsychopharmacology · 2024Article
- Article
- Diminishing neuronal acidification by channelrhodopsins with low proton conduction.bioRxiv : the preprint server for biology · 2023Article
- Video-based pooled screening yields improved far-red genetically encoded voltage indicators.Nature methods · 2023Article
- Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types.Science (New York, N.Y.) · 2022Article
- Automatic Multi-functional Integration Program (AMFIP) towards all-optical mechano-electrophysiology interrogation.PloS one · 2022Article
- Highly Parallelized, Multicolor Optogenetic Recordings of Cellular Activity for Therapeutic Discovery Applications in Ion Channels and Disease-Associated Excitable Cells.Frontiers in molecular neuroscience · 2022Article
- Rhodopsin-Based Optogenetics: Basics and Applications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Linearly polarized excitation enhances signals from fluorescent voltage indicators.Biophysical journal · 2021Article
- Surface tension and viscosity of protein condensates quantified by micropipette aspiration.Biophysical reports · 2021Article
- A high-content platform for physiological profiling and unbiased classification of individual neurons.Cell reports methods · 2021Article
- Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.Science advances · 2021Article
- Imaging Voltage with Microbial Rhodopsins.Frontiers in molecular biosciences · 2021Review
- Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.International journal of molecular sciences · 2020Review
- Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain.International journal of molecular medicine · 2020Article
- The Next 50 Years of Neuroscience.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Voltage-gated ion channels mediate electrical dynamics in excitable tissues and are an important class of drug targets. Channels can gate in sub-millisecond timescales, show complex manifolds of conformational states, and often show state-dependent pharmacology. Mechanistic studies of ion channels typically involve sophisticated voltage-clamp protocols applied through manual or automated electrophysiology. Here, we develop all-optical electrophysiology techniques to study activity-dependent modulation of ion channels, in a format compatible with high-throughput screening. Using optical electrophysiology, we recapitulate many voltage-clamp protocols and apply to Nav1.7, a channel implicated in pain. Optical measurements reveal that a sustained depolarization strongly potentiates the inhibitory effect of PF-04856264, a Nav1.7-specific blocker. In a pilot screen, we stratify a library of 320 FDA-approved compounds by binding mechanism and kinetics, and find close concordance with patch clamp measurements. Optical electrophysiology provides a favorable tradeoff between throughput and information content for studies of NaV channels, and possibly other voltage-gated channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.