ArticleBiotechnology and bioengineering2016
Genome-scale RNA interference screen identifies antizyme 1 (OAZ1) as a target for improvement of recombinant protein production in mammalian cells.
Article in Biotechnology and bioengineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Cell Engineering for Increasing Production of Recombinant Proteins in Mammalian Cells.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026Review
- A CRISPR-HITI strategy approach to improve CHO cell viability by modifying the 3'UTR of Caspase 8 Associated Protein 2.Molecular biology research communications · 2025Article
- Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes.Cells · 2021Review
- From omics to Cellular mechanisms in mammalian cell factory development.Current opinion in chemical engineering · 2021Article
- Knockout of the caspase 8-associated protein 2 gene improves recombinant protein expression in HEK293 cells through up-regulation of the cyclin-dependent kinase inhibitor 2A gene.Biotechnology and bioengineering · 2021Article
- BRCA1 and S phase DNA repair pathways restrict LINE-1 retrotransposition in human cells.Nature structural & molecular biology · 2020Article
- Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1.New biotechnology · 2020Article
- A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets.Scientific reports · 2019Article
- Knocking out Ornithine Decarboxylase Antizyme 1 (Medical sciences (Basel, Switzerland) · 2018Article
- Identifying HIPK1 as Target of miR-22-3p Enhancing Recombinant Protein Production From HEK 293 Cell by Using Microarray and HTP siRNA Screen.Biotechnology journal · 2018Article
- Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells.Genes · 2018Review
- Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Genetic Architecture and Candidate Genes Identified for Follicle Number in Chicken.Scientific reports · 2017Article
- OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese.PloS one · 2017Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
For the purpose of improving recombinant protein production from mammalian cells, an unbiased, high-throughput whole-genome RNA interference screen was conducted using human embryonic kidney 293 (HEK 293) cells expressing firefly luciferase. A 21,585 human genes were individually silenced with three different siRNAs for each gene. The screen identified 56 genes that led to the greatest improvement in luciferase expression. These genes were found to be included in several pathways involved in spliceosome formation and mRNA processing, transcription, metabolic processes, transport, and protein folding. The 10 genes that most enhanced protein expression when downregulated, were further confirmed by measuring the effect of their silencing on the expression of three additional recombinant proteins. Among the confirmed genes, OAZ1-the gene encoding the ornithine decarboxylase antizyme1-was selected for detailed investigation, since its silencing improved the reporter protein production without affecting cell viability. Silencing OAZ1 caused an increase of the ornithine decarboxylase enzyme and the cellular levels of putrescine and spermidine; an indication that increased cellular polyamines enhances luciferase expression without affecting its transcription. The study shows that OAZ1 is a novel target for improving expression of recombinant proteins. The genome-scale screening performed in this work can establish the foundation for targeted design of an efficient mammalian cell platform for various biotechnological applications. Biotechnol. Bioeng. 2016;113: 2403-2415. © 2016 Wiley Periodicals, Inc.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.