ArticleThe EMBO journal1989
The contact site A glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type.
Article in The EMBO journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
26 citing papers in PubMed, 111 citations in OpenAlex.
- Aberrant adhesion impacts early development in a Dictyostelium model for juvenile neuronal ceroid lipofuscinosis.Cell adhesion & migration · 2017Article
- The Dictyostelium discoideum GPHR ortholog is an endoplasmic reticulum and Golgi protein with roles during development.Eukaryotic cell · 2015Article
- The control of chemotactic cell movement during Dictyostelium morphogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Adhesion-induced receptor segregation and adhesion plaque formation: A model membrane study.Biophysical journal · 1999Article
- Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis.The Journal of cell biology · 1997Article
- Lipid remodeling leads to the introduction and exchange of defined ceramides on GPI proteins in the ER and Golgi of Saccharomyces cerevisiae.The EMBO journal · 1997Article
- Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.Molecular biology of the cell · 1997Article
- A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex.Molecular and cellular biology · 1996Article
- An uncleaved glycosylphosphatidylinositol signal mediates Ca(2+)-sensitive protein degradation.The Biochemical journal · 1996Article
- Cell adhesion in the life cycle of Dictyostelium.Experientia · 1995Review
- Glycosylinositol-phosphoceramide in the free-living protozoan Paramecium primaurelia: modification of core glycans by mannosyl phosphate.The EMBO journal · 1995Article
- Biosynthesis of glycosylphosphatidylinositol membrane anchors.The Biochemical journal · 1995Review
- Temperature-induced alteration of inositolphosphorylceramides in the putative glycosylated lipid precursors of Tetrahymena mimbres glycosylphosphatidylinositol-anchored proteins.The Biochemical journal · 1995Article
- Lack of glycosyl-phosphatidylinositol anchoring leads to precursor retention by a unique mechanism in Dictyostelium discoideum.The Biochemical journal · 1995Article
- Ponticulin is an atypical membrane protein.The Journal of cell biology · 1994Article
- Addition of lipid substituents of mammalian protein glycosylphosphoinositol anchors.Molecular and cellular biology · 1994Article
- Replacement of the phospholipid-anchor in the contact site A glycoprotein of D. discoideum by a transmembrane region does not impede cell adhesion but reduces residence time on the cell surface.The Journal of cell biology · 1994Article
- The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes.The Biochemical journal · 1993Review
- Size polymorphisms due to changes in the number of O-glycosylated tandem repeats in the Dictyostelium discoideum glycoprotein PsA.Genetics · 1992Article
- Two different types of lipid moieties are present in glycophosphoinositol-anchored membrane proteins of Saccharomyces cerevisiae.The EMBO journal · 1992Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The contact site A glycoprotein, a cell adhesion protein of aggregating Dictyostelium cells, was labeled with fatty acid, myo-inositol, phosphate and ethanolamine in vivo, indicating that the protein is anchored in the membrane by a lipid. This lipid was not susceptible to phosphatidyl inositol specific phospholipase C. When cleaved with nitrous acid or when subjected to acetolysis, the anchor released lipids which were different from those released from Trypanosoma variant cell surface glycoprotein, a protein with a known phosphatidyl inositol-glycan anchor. Resistance to weak and sensitivity to strong alkali indicated that the fatty acid in the contact site A glycolipid anchor was in an amide bond. On incubation with sphingomyelinase, a lipid with the chromatographic behavior of ceramide was released. These results suggest that the contact site A glycoprotein is anchored by a ceramide based lipid glycan.
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Registered trials
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