ArticleJournal of visualized experiments : JoVE2016
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Multiplexed fluorescence and scatter detection with single cell resolution using on-chip fiber optics for droplet microfluidic applications.Microsystems & nanoengineering · 2024Article
- Recent advances in droplet sequential monitoring methods for droplet sorting.Biomicrofluidics · 2023Review
- spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content.Nature communications · 2023Article
- Droplet Detection and Sorting System in Microfluidics: A Review.Micromachines · 2022Review
- Droplet Microfluidic Device for Chemoenzymatic Sensing.Micromachines · 2022Article
- Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.Biotechnology journal · 2022Article
- A microfluidic flow analyzer with integrated lensed optical fibers.Biomicrofluidics · 2020Article
- Optofluidic detection setup for multi-parametric analysis of microbiological samples in droplets.Biomicrofluidics · 2020Article
- Recent Advances in Design of Fluorescence-Based Assays for High-Throughput Screening.Analytical chemistry · 2019Review
- 3D-glass molds for facile production of complex droplet microfluidic chips.Biomicrofluidics · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Fluorescence assays are the most common readouts used in droplet microfluidics due to their bright signals and fast time response. Applications such as multiplex assays, enzyme evolution, and molecular biology enhanced cell sorting require the detection of two or more colors of fluorescence. Standard multicolor detection systems that couple free space lasers to epifluorescence microscopes are bulky, expensive, and difficult to maintain. In this paper, we describe a scheme to perform multicolor detection by exciting discrete regions of a microfluidic channel with lasers coupled to optical fibers. Emitted light is collected by an optical fiber coupled to a single photodetector. Because the excitation occurs at different spatial locations, the identity of emitted light can be encoded as a temporal shift, eliminating the need for more complicated light filtering schemes. The system has been used to detect droplet populations containing four unique combinations of dyes and to detect sub-nanomolar concentrations of fluorescein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.