ArticleThe New phytologist2016
Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids.
Article in The New phytologist, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 92 citations in OpenAlex.
- Diverse oxidosqualene cyclases for triterpenoid biosynthesis.Synthetic and systems biotechnology · 2027Review
- Evaluation of a high throughput multiparallel stirred bioreactor system using an apple cell line.Scientific reports · 2026Article
- Harnessing Apple Cell Suspension Cultures in Bioreactors for Triterpene Production: Transcriptomic Insights into Biomass and Triterpene Biosynthesis.International journal of molecular sciences · 2025Article
- Elicitor-mediated simultaneous accumulation of phloridzin and ursolic acid in Annurca apple peel-derived calli.Journal of the science of food and agriculture · 2025Article
- Progress in the study of bioactivity, chemical composition and pharmacological mechanism of action inFrontiers in pharmacology · 2025Review
- Recent trends in the elucidation of complex triterpene biosynthetic pathways in horticultural trees.Horticulture research · 2025Article
- Roles of three cytochrome P450 monooxygenases in triterpene biosynthesis and their potential impact on growth and development.Plant physiology · 2024Article
- Anti-Microorganisms · 2024Article
- Impact of Cytochrome P450 Enzyme on Fruit Quality.International journal of molecular sciences · 2024Review
- Integrated Metabolomics and Transcriptomics Analysis Reveals New Insights into Triterpene Biosynthesis inPlants (Basel, Switzerland) · 2024Article
- Triterpene tales: Two genes involved in Nicotiana attenuata herbivore defense.Plant physiology · 2024Article
- OXIDOSQUALENE CYCLASE 1 and 2 influence triterpene biosynthesis and defense in Nicotiana attenuata.Plant physiology · 2024Article
- Cuticular wax biosynthesis in blueberries (Horticulture research · 2024Article
- Beyond skin-deep: targeting the plant surface for crop improvement.Journal of experimental botany · 2023Review
- Hydroxylases involved in terpenoid biosynthesis: a review.Bioresources and bioprocessing · 2023Review
- OeBAS and CYP716C67 catalyze the biosynthesis of health-beneficial triterpenoids in olive (Olea europaea) fruits.The New phytologist · 2023Article
- Triterpenoids of Three Apple Cultivars-Biosynthesis, Antioxidative and Anti-Inflammatory Properties, and Fate during Processing.Molecules (Basel, Switzerland) · 2023Article
- Applications of protein engineering in the microbial synthesis of plant triterpenoids.Synthetic and systems biotechnology · 2023Review
- Role of Cytochrome P450 Enzyme in Plant Microorganisms' Communication: A Focus on Grapevine.International journal of molecular sciences · 2023Review
- Molecular Characterization of anMolecules (Basel, Switzerland) · 2022Article
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apple (Malus × domestica) accumulates bioactive ursane-, oleanane-, and lupane-type triterpenes in its fruit cuticle, but their biosynthetic pathway is still poorly understood. We used a homology-based approach to identify and functionally characterize two new oxidosqualene cyclases (MdOSC4 and MdOSC5) and one cytochrome P450 (CYP716A175). The gene expression patterns of these enzymes and of previously described oxidosqualene cyclases were further studied in 20 apple cultivars with contrasting triterpene profiles. MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as β-amyrin and lupeol, in the proportion 82 : 14 : 4. MdOSC5 cyclizes 2,3-oxidosqualene into lupeol and β-amyrin at a ratio of 95 : 5. CYP716A175 catalyses the C-28 oxidation of α-amyrin, β-amyrin, lupeol and germanicol, producing ursolic acid, oleanolic acid, betulinic acid, and putatively morolic acid. The gene expression of MdOSC1 was linked to the concentrations of ursolic and oleanolic acid, whereas the expression of MdOSC5 was correlated with the concentrations of betulinic acid and its caffeate derivatives. Two new multifuntional triterpene synthases as well as a multifunctional triterpene C-28 oxidase were identified in Malus × domestica. This study also suggests that MdOSC1 and MdOSC5 are key genes in apple fruit triterpene biosynthesis.
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