ArticleBiotechnology for biofuels2016
Exploring fatty alcohol-producing capability of Yarrowia lipolytica.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 86 citations in OpenAlex.
- Unique trajectory of gene family evolution from genomic analysis of nearly all known species in an ancient yeast lineage.Molecular systems biology · 2025Article
- Single-cell protein production from COEnvironmental science and ecotechnology · 2025Article
- Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast.Journal of biological engineering · 2024Review
- High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis inACS synthetic biology · 2023Article
- Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeastProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A paradigm shift towards production of sustainable bioenergy and advanced products fromBiomass conversion and biorefinery · 2022Review
- Non-homologous End Joining-Mediated Insertional Mutagenesis Reveals a Novel Target for Enhancing Fatty Alcohols Production inFrontiers in microbiology · 2022Article
- Microbial engineering to produce fatty alcohols and alkanes.Journal of industrial microbiology & biotechnology · 2021Review
- Metabolic Engineering of Non-carotenoid-Producing YeastFrontiers in microbiology · 2021Article
- Elevated β-Carotene Production Using Codon-Adapted CarRA&B and Metabolic Balance in EngineeredFrontiers in microbiology · 2021Article
- Engineering the oleaginous yeast Yarrowia lipolytica for high-level resveratrol production.Metabolic engineering · 2020Article
- Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform.Journal of industrial microbiology & biotechnology · 2020Review
- Direct production of fatty alcohols from glucose using engineered strains ofMetabolic engineering communications · 2020Article
- Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations.Frontiers in bioengineering and biotechnology · 2020Review
- Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis inFrontiers in microbiology · 2020Article
- Promoting the Synthesis of Precursor Substances by Overexpressing Hexokinase (Hxk) and Hydroxymethylglutaryl-CoA Synthase (Erg13) to Elevate β-Carotene Production in EngineeredFrontiers in microbiology · 2020Article
- Metabolic Engineering of Oleaginous YeastFrontiers in microbiology · 2020Article
- Review
- Production of Long Chain Fatty Alcohols Found in Bumblebee Pheromones byFrontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFatty alcohols are important oleochemicals widely used in detergents, surfactants and personal care products. Bio-synthesized fatty alcohol provides a promising alternative to traditional fatty alcohol industry. Harnessing oleaginous microorganisms for fatty alcohol production may offer a new strategy to achieve a commercially viable yield that currently still seems to be a remote target.
resultsIn this study, we introduced functional fatty acyl-CoA reductase (FAR), TaFAR1 to direct the conversion from fatty acyl-CoA to fatty alcohol in Yarrowia lipolytica (Y. lipolytica), an oleaginous non-conventional yeast showing great lipid-producing capability. Tri-module optimizations including eliminating fatty alcohol degradation pathway, enhancing TaFAR1 expression, and increasing fatty acyl-CoA supply were furtherly conducted, resulting in 63-fold increase in intracellular fatty alcohol-producing capability compared to the starting strain. Thus, this work demonstrated successful construction of first generation of Y. lipolytica fatty alcohol-producing cell factory. Through the study of effect of environmental nutrition on fatty alcohol production, up to 636.89 mg/L intracellular hexadecanol (high fatty alcohol-retaining capability) and 53.32 mg/L extracellular hexadecanol were produced by this cell factory through batch fermentation, which was comparable to the highest production of Saccharomyces cerevisiae under the similar condition.
conclusionThis work preliminarily explored fatty alcohol-producing capability through mobilization of FAR and fatty acid metabolism, maximizing the intracellular fatty alcohol-producing capability, suggesting that Y. lipolytica cell factory potentially offers a promising platform for fatty alcohol production.
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