ArticleScientific reports2016
Efficient selective breeding of live oil-rich Euglena gracilis with fluorescence-activated cell sorting.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 103 citations in OpenAlex.
- A low temperature-adaptedApplied and environmental microbiology · 2026Article
- In-air microfluidic sorting of single cells on multiple paths.Microsystems & nanoengineering · 2025Article
- Characterization ofMicroorganisms · 2025Article
- Spatiotemporally controlled microvortices provide advanced microfluidic components.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation.Scientific reports · 2024Article
- Zeaxanthin is required for eyespot formation and phototaxis in Euglena gracilis.Plant physiology · 2023Article
- Developing algae as a sustainable food source.Frontiers in nutrition · 2022Review
- Article
- Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme.Communications biology · 2021Article
- Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter.Science advances · 2021Article
- From Classical Radiation to Modern Radiation: Past, Present, and Future of Radiation Mutation Breeding.Frontiers in public health · 2021Review
- Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria.Advances in experimental medicine and biology · 2021Article
- Noninvasive and Safe Cell Viability Assay for Breast Cancer MCF-7 Cells Using Natural Food Pigment.Biology · 2020Article
- Positive dielectrophoresis-based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo.Science advances · 2020Article
- Noninvasive and safe cell viability assay for Paramecium using natural pigment extracted from food.Scientific reports · 2020Article
- Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.Nature communications · 2020Article
- Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling.RSC advances · 2020Article
- Large-scale label-free single-cell analysis of paramylon inBiomedical optics express · 2020Article
- Genetic Engineering Strategies forFrontiers in bioengineering and biotechnology · 2020Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Euglena gracilis, a microalgal species of unicellular flagellate protists, has attracted much attention in both the industrial and academic sectors due to recent advances in the mass cultivation of E. gracilis that have enabled the cost-effective production of nutritional food and cosmetic commodities. In addition, it is known to produce paramylon (β-1,3-glucan in a crystalline form) as reserve polysaccharide and convert it to wax ester in hypoxic and anaerobic conditions-a promising feedstock for biodiesel and aviation biofuel. However, there remain a number of technical challenges to be solved before it can be deployed in the competitive fuel market. Here we present a method for efficient selective breeding of live oil-rich E. gracilis with fluorescence-activated cell sorting (FACS). Specifically, the selective breeding method is a repetitive procedure for one-week heterotrophic cultivation, staining intracellular lipids with BODIPY(505/515), and FACS-based isolation of top 0.5% lipid-rich E. gracilis cells with high viability, after inducing mutation with Fe-ion irradiation to the wild type (WT). Consequently, we acquire a live, stable, lipid-rich E. gracilis mutant strain, named B1ZFeL, with 40% more lipid content on average than the WT. Our method paves the way for rapid, cost-effective, energy-efficient production of biofuel.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.