Shedding light on betL: pPL2-lux mediated real-time analysis of betL expression in Listeria monocytogenes.

Shauna M Keane, Eamonn P Culligan, Roland F Hoffmann, Cormac G M Gahan, Colin Hill, William J Snelling, Roy D Sleator

Abstract readComment

In one paragraph

Article in Bioengineered, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Comment on
A single point mutation in the listerial betL σ(A)-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity.

5 · Who and what money

Authors and funding

7 authors.

Shauna M Keanea Department of Biological Sciences , Cork Institute of Technology , Rossa Avenue, Bishopstown, Cork , Ireland.

Eamonn P Culligana Department of Biological Sciences , Cork Institute of Technology , Rossa Avenue, Bishopstown, Cork , Ireland.

Roland F Hoffmannb APC Microbiome Institute, University College Cork , College Road, Cork , Ireland.

Cormac G M Gahanb APC Microbiome Institute, University College Cork , College Road, Cork , Ireland.

Colin Hillb APC Microbiome Institute, University College Cork , College Road, Cork , Ireland.

William J Snellinge Biomedical Sciences Research Institute, Ulster University , Northern Ireland.

Roy D Sleatora Department of Biological Sciences , Cork Institute of Technology , Rossa Avenue, Bishopstown, Cork , Ireland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*.

Indexed as

Gene Expression Regulation, BacterialBacterial ProteinsBetaineCarrier ProteinsListeria monocytogenesBacterial ProteinsBetaineCarrier ProteinsbetL*Listerialuxosmotolerance

Identifiers

PMID27212260

DOI10.1080/21655979.2016.1171438

PMCPMC4879987

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.