ArticleScientific reports2016
Interrupting peptidoglycan deacetylation during Bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Comparison of Predatory Phenotypes and Genotypes Between Bdellovibrio sp. BIS2 and Bacteriovorax sp. HI3 Isolated From the Same Freshwater Environment.Environmental microbiology · 2026Article
- Review
- A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus.PLoS genetics · 2025Article
- Quantitative proteome of bacterial periplasmic predation by Bdellovibrio bacteriovorus reveals a prey-lytic protease.Communications biology · 2025Article
- Structural and functional analysis of Pseudomonas aeruginosa PelA provides insight into the modification of the Pel exopolysaccharide.The Journal of biological chemistry · 2025Article
- Article
- How do Gram-negative bacteria escape predation by Bdellovibrio bacteriovorus?npj antimicrobials and resistance · 2024Review
- Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin.Nature communications · 2024Article
- Bdellovibrio bacteriovorus uses chimeric fibre proteins to recognize and invade a broad range of bacterial hosts.Nature microbiology · 2024Article
- Two-state swimming: Strategy and survival of a model bacterial predator in response to environmental cues.Biophysical journal · 2023Article
- Moving toward a better understanding of the model bacterial predatorMicrobiology (Reading, England) · 2023Article
- Learning with Bdellovibrio.Nature microbiology · 2023Article
- Bdellovibrio predation cycle characterized at nanometre-scale resolution with cryo-electron tomography.Nature microbiology · 2023Article
- Binary or Nonbinary Fission? Reproductive Mode of a Predatory Bacterium Depends on Prey Size.mBio · 2023Article
- An MltA-Like Lytic Transglycosylase Secreted by Bdellovibrio bacteriovorus Cleaves the Prey Septum during Predatory Invasion.Journal of bacteriology · 2023Article
- Advances in cellular and molecular predatory biology ofFrontiers in microbiology · 2023Review
- Crystal structure of ChbG from Klebsiella pneumoniae reveals the molecular basis of diacetylchitobiose deacetylation.Communications biology · 2022Article
- Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates.Scientific reports · 2022Article
- Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria.Nature communications · 2022Article
- A Dynamic Network of Proteins Facilitate Cell Envelope Biogenesis in Gram-Negative Bacteria.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
The peptidoglycan wall, located in the periplasm between the inner and outer membranes of the cell envelope in Gram-negative bacteria, maintains cell shape and endows osmotic robustness. Predatory Bdellovibrio bacteria invade the periplasm of other bacterial prey cells, usually crossing the peptidoglycan layer, forming transient structures called bdelloplasts within which the predators replicate. Prey peptidoglycan remains intact for several hours, but is modified and then degraded by escaping predators. Here we show predation is altered by deleting two Bdellovibrio N-acetylglucosamine (GlcNAc) deacetylases, one of which we show to have a unique two domain structure with a novel regulatory"plug". Deleting the deacetylases limits peptidoglycan degradation and rounded prey cell "ghosts" persist after mutant-predator exit. Mutant predators can replicate unusually in the periplasmic region between the peptidoglycan wall and the outer membrane rather than between wall and inner-membrane, yet still obtain nutrients from the prey cytoplasm. Deleting two further genes encoding DacB/PBP4 family proteins, known to decrosslink and round prey peptidoglycan, results in a quadruple mutant Bdellovibrio which leaves prey-shaped ghosts upon predation. The resultant bacterial ghosts contain cytoplasmic membrane within bacteria-shaped peptidoglycan surrounded by outer membrane material which could have promise as "bacterial skeletons" for housing artificial chromosomes.
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Registered trials
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