ArticleBioanalysis2016
Development and validation of an HILIC-MS/MS method by one-step precipitation for chloroquine in miniature pig plasma.
Article in Bioanalysis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- A Simple and Sensitive HPLC-MS/MS Assay for the Quantitation of Blonanserin and N-Desethyl Blonanserin in Rat Plasma and Its Application to Pharmacokinetic Study.Journal of analytical methods in chemistry · 2022Article
- Liquid Chromatographic Methods for COVID-19 Drugs, Hydroxychloroquine and Chloroquine.Journal of chromatographic science · 2021Review
- HPLC methods for choloroquine determination in biological samples and pharmaceutical products.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantification of polar compounds such as chloroquine by revered-phase LC is a challenge because of poor retention and silanol interactions with stationary phase. Strong ion-pairing reagents added to mobile phases to improve reversed-phase retention and improve peak shape can be harmful for MS.
resultsThis new approach provides a rapid and sensitive method for the detection of chloroquine using hydrophilic interaction LC coupled to MS/MS (HILIC-MS/MS). Ammonium formate and formic acid were added to mobile phase to attain good peak shapes and the salified chloroquine as well retained in an HILIC column. Linearity, intra- and inter-day precision, accuracy, recovery, matrix effect and stability were evaluated during the validation process.
conclusionThe validated method has been successfully used in a PK study in miniature pigs, and paves way for future development.
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