ArticleScientific reports2016
Decellularization of human stromal refractive lenticules for corneal tissue engineering.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 2 syntheses or guidelines pooled it, 87 citations in OpenAlex.
- Femtosecond laser-assisted implantation of corneal stroma lenticule for keratoconus.International ophthalmology · 2021Pooled it
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- Preservation and Reuse of KLEx Lenticules: From Biobanking to Therapeutic and Refractive Innovations.Bioengineering (Basel, Switzerland) · 2026Review
- Biological assessment and preservation strategies for SMILE- derived lenticules: towards a corneal regenerative biobank.Scientific reports · 2026Article
- Decellularized corneal-based 3D scaffolds: methods decellularization, characterization, mechanical properties, and species source.Regenerative therapy · 2026Review
- Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening.Journal of materials science. Materials in medicine · 2025Article
- Evaluation ofFrontiers in bioengineering and biotechnology · 2025Article
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities.Bioactive materials · 2024Review
- Fabrication of bioengineered corneal endothelial grafts using an allogeneic cornea-derived matrix.Materials today. Bio · 2024Review
- Regenerative Therapy for Corneal Scarring Disorders.Biomedicines · 2024Review
- Effects of mesenchymal stromal cells and human recombinant Nerve Growth Factor delivered by bioengineered human corneal lenticule on an innovative model of diabetic retinopathy.Frontiers in endocrinology · 2024Article
- Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature.Journal of ophthalmology · 2024Review
- Applications of SMILE-extracted lenticules in ophthalmology.International journal of ophthalmology · 2024Review
- Visual outcomes and corneal densitometry after allogenic and autologous lenticule intrastromal keratoplasty for the correction of moderate-to-high hyperopia.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023Article
- A composite hydrogel membrane with shape and water retention for corneal tissue engineering.Heliyon · 2023Article
- Functional acellular matrix for tissue repair.Materials today. Bio · 2023Review
- Preservation of corneal stromal lenticule: review.Cell and tissue banking · 2022Review
- Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives.International journal of molecular sciences · 2022Review
- Effect of corneal stromal lenticule customization on neurite distribution and excitatory property.Journal of advanced research · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small incision lenticule extraction (SMILE) becomes a procedure to correct myopia. The extracted lenticule can be used for other clinical scenarios. To prepare for allogeneic implantation, lenticule decellularization with preserved optical property, stromal architecture and chemistry would be necessary. We evaluated different methods to decellularize thin human corneal stromal lenticules created by femtosecond laser. Treatment with 0.1% sodium dodecylsulfate (SDS) followed by extensive washes was the most efficient protocol to remove cellular and nuclear materials. Empty cell space was found inside the stroma, which displayed aligned collagen fibril architecture similar to native stroma. The SDS-based method was superior to other treatments with hyperosmotic 1.5 M sodium chloride, 0.1% Triton X-100 and nucleases (from 2 to 10 U/ml DNase and RNase) in preserving extracellular matrix content (collagens, glycoproteins and glycosaminoglycans). The stromal transparency and light transmittance was indifferent to untreated lenticules. In vitro recellularization showed that the SDS-treated lenticules supported corneal stromal fibroblast growth. In vivo re-implantation into a rabbit stromal pocket further revealed the safety and biocompatibility of SDS-decellularized lenticules without short- and long-term rejection risk. Our results concluded that femtosecond laser-derived human stromal lenticules decellularized by 0.1% SDS could generate a transplantable bioscaffold with native-like stromal architecture and chemistry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.