ArticleScientific reports2016
Selection of Reference Genes for Gene Expression Studies related to lung injury in a preterm lamb model.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Reference gene screening for analyzing gene expression in the heart, liver, spleen, lung and kidney of forest musk deer.The Journal of veterinary medical science · 2021Article
- Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury.International journal of molecular sciences · 2021Article
- Exosomal Long Non-Coding RNAs in Lung Diseases.International journal of molecular sciences · 2020Review
- Article
- Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.Molecular biology reports · 2018Article
- Plasma proteomics reveals gestational age-specific responses to mechanical ventilation and identifies the mechanistic pathways that initiate preterm lung injury.Scientific reports · 2018Article
- Overexpression of theInternational journal of molecular sciences · 2018Article
- Selection and validation of suitable reference genes for qRT-PCR analysis in pear leaf tissues under distinct training systems.PloS one · 2018Article
- Lung Recruitment Strategies During High Frequency Oscillatory Ventilation in Preterm Lambs.Frontiers in pediatrics · 2018Article
- Identification of reference genes and miRNAs for qRT-PCR in human esophageal squamous cell carcinoma.Medical oncology (Northwood, London, England) · 2017Article
- Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin.PloS one · 2017Article
- Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restriction.Scientific reports · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preterm newborns often require invasive support, however even brief periods of supported ventilation applied inappropriately to the lung can cause injury. Real-time quantitative reverse transcriptase-PCR (qPCR) has been extensively employed in studies of ventilation-induced lung injury with the reference gene 18S ribosomal RNA (18S RNA) most commonly employed as the internal control reference gene. Whilst the results of these studies depend on the stability of the reference gene employed, the use of 18S RNA has not been validated. In this study the expression profile of five candidate reference genes (18S RNA, ACTB, GAPDH, TOP1 and RPS29) in two geographical locations, was evaluated by dedicated algorithms, including geNorm, Normfinder, Bestkeeper and ΔCt method and the overall stability of these candidate genes determined (RefFinder). Secondary studies examined the influence of reference gene choice on the relative expression of two well-validated lung injury markers; EGR1 and IL1B. In the setting of the preterm lamb model of lung injury, RPS29 reference gene expression was influenced by tissue location; however we determined that individual ventilation strategies influence reference gene stability. Whilst 18S RNA is the most commonly employed reference gene in preterm lamb lung studies, our results suggest that GAPDH is a more suitable candidate.
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