ArticleNano letters2016
Cooperative Vinculin Binding to Talin Mapped by Time-Resolved Super Resolution Microscopy.
Article in Nano letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Mechanical Signaling From ECMs to Nuclear Envelopes in Cell Transitions and Fate Decisions: A Review and Perspective on Adhesion-Niche-Based Anti-EndMT and Anti-EMT.Annals of biomedical engineering · 2026Review
- Molecular dynamics simulations reveal how vinculin refolds partially unfolded talin rod helices to stabilize them against mechanical force.PLoS computational biology · 2024Article
- Differential Talin cleavage in transformed and non-transformed cells and its consequences.Frontiers in cell and developmental biology · 2024Article
- Kindlin stabilizes the talin·integrin bond under mechanical load by generating an ideal bond.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions.Nature reviews. Molecular cell biology · 2023Review
- Plasma Concentrations of Vinculin versus Talin-1 in Coronary Artery Disease.Medical sciences (Basel, Switzerland) · 2022Article
- Genetic Code Expansion: A Brief History and Perspective.Biochemistry · 2021Review
- Real-time imaging of cellular forces using optical interference.Nature communications · 2021Article
- Article
- Molecular Tension Sensors: Moving Beyond Force.Current opinion in biomedical engineering · 2019Article
- Integrin nanoclusters can bridge thin matrix fibres to form cell-matrix adhesions.Nature materials · 2019Article
- Talin1 knockdown prohibits the proliferation and migration of colorectal cancer cells via the EMT signaling pathway.Oncology letters · 2019Article
- Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.Nature communications · 2019Article
- Steps in Mechanotransduction Pathways that Control Cell Morphology.Annual review of physiology · 2019Review
- Talin as a mechanosensitive signaling hub.The Journal of cell biology · 2018Review
- Single and collective cell migration: the mechanics of adhesions.Molecular biology of the cell · 2017Article
- Molecular stretching modulates mechanosensing pathways.Protein science : a publication of the Protein Society · 2017Review
- Downregulation of Talin1 promotes hepatocellular carcinoma progression through activation of the ERK1/2 pathway.Cancer science · 2017Article
- Force Bistability in Adhesion Switch.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The dimeric focal adhesion protein talin contains up to 22 cryptic vinculin binding sites that are exposed by unfolding. Using a novel method to monitor the in situ dynamics of the talin dimer stretch, we find that in contrast to several prevalent talin dimer models the integrin-binding talin N-termini are separated by 162 ± 44 nm on average whereas as expected the C-terminal dimerization domains colocalize and are mobile. Using vinculin tagged by DHFR-TMP Atto655 label, we found that optimal vinculin and vinculin head binding occurred when talin was stretched to 180 nm, while the controls did not bind to talin. Surprisingly, multiple vinculins bound within a single second in narrowly localized regions of the talin rod during stretching. We suggest that talin stretches as an antiparallel dimer and that activates vinculin binding in a cooperative manner, consistent with the stabilization of folded talin by other binding proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.