ArticleAnalytical and bioanalytical chemistry2016
SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological material.
Article in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Electrochemical biosensing in oncology: a review advancements and prospects for cancer diagnosis.Cancer biology & therapy · 2025Review
- Is Osteopontin a Reliable Biomarker for Endometriosis?International journal of molecular sciences · 2024Article
- Application of Surface Plasmon Resonance Imaging Biosensors for Determination of Fibronectin, Laminin-5, and Type IV Collagen in Plasma, Urine, and Tissue of Renal Cell Carcinoma.Sensors (Basel, Switzerland) · 2024Article
- Is the leptin/BMI ratio a reliable biomarker for endometriosis?Frontiers in endocrinology · 2024Article
- The Levels of Leptin, Cystatin C, Neuropilin-1 and Tau Protein in Relation to Dietary Habits in Patients with Alzheimer's Disease.Journal of clinical medicine · 2023Article
- Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis.International journal of molecular sciences · 2023Article
- Review
- An Array SPRi Biosensor for the Determination on PARP-1 in Blood Plasma.Biomedicines · 2023Article
- Application of biosensors in cancers, an overview.Frontiers in bioengineering and biotechnology · 2023Review
- Plasma and Peritoneal Fluid Fibronectin and Collagen IV Levels as Potential Biomarkers of Endometriosis.International journal of molecular sciences · 2022Article
- Laminin-5, Fibronectin, and Type IV Collagen as Potential Biomarkers of Brain Glioma Malignancy.Biomedicines · 2022Article
- Two Biosensors for the Determination of Interleukin-6 in Blood Plasma by Array SPRi.Biosensors · 2022Article
- UCHL1 and Proteasome in Blood Serum in Relation to Dietary Habits, Concentration of Selected Antioxidant Minerals and Total Antioxidant Status among Patients with Alzheimer's Disease.Journal of clinical medicine · 2022Article
- Bioanalytical methods for circulating extracellular matrix-related proteins: new opportunities in cancer diagnosis.Analytical and bioanalytical chemistry · 2022Review
- Article
- An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma.Sensors (Basel, Switzerland) · 2021Article
- Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-α5 Gene Expression in Balb/C Mouse Seminiferous Tubules.Galen medical journal · 2019Article
- Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new method for the selective determination of laminin-5 concentration using a biosensor and surface plasmon resonance imaging (SPRI) technique is presented. A biosensor based on the specific interaction of laminin-5 with rabbit polyclonal antibody was constructed. The analytically useful dynamic response range of the biosensor is between 0.014 and 0.1 ng mL(-1). The detection limit is 4 pg mL(-1). The potential influence of interferences on the SPRI signal was investigated, and the high selectivity of the biosensor was confirmed. In order to demonstrate the potential application of the biosensor, laminin-5 concentration in blood plasma was determined. The results were compared with the laminin-5 concentration obtained by the commercial enzyme-linked immunosorbent assay (ELISA) kit. A comparison of results from healthy donors obtained by SPRI measurement and ELISA indicates that they are close and shows good agreement with the data reported in the literature. The plasma samples of bladder cancer patients gave higher concentration measured with specific biosensor than by ELISA assay. The study shows the clear difference in concentration of laminin-5 in healthy humans and patients with bladder cancer. Extensive clinical studies using the newly developed method can result in an increase in the use of laminin-5 as a potential cancer marker.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.