ArticleRNA (New York, N.Y.)2016
The piRNA pathway is developmentally regulated during spermatogenesis in Drosophila.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- The impact of P-element-induced hybrid dysgenesis on the male germline in Drosophila simulans.Genetics · 2026Article
- Sex-biased Expression of Genes and Transposable Elements in Hybrids from Drosophila mojavensis and Drosophila arizonae.Genome biology and evolution · 2026Article
- Sex-stratified piRNA expression analysis reveals shared functional impacts of perinatal lead (Pb) exposure in murine hearts.Epigenetics · 2025Article
- Abundant A-to-I RNA editing in spermatocytes may suppress transposons to compensate for piRNA downregulation in male germlines.RNA biology · 2025Article
- Single-cell consequences of X-linked meiotic drive in stalk-eyed flies.PLoS genetics · 2025Article
- piRNA processing within non-membrane structures is governed by constituent proteins and their functional motifs.The FEBS journal · 2025Review
- Regulatory roles of PIWI-interacting RNAs in cardiovascular disease.American journal of physiology. Heart and circulatory physiology · 2025Review
- Non-gonadal expression of piRNAs is widespread across Arthropoda.FEBS letters · 2025Review
- The functions and mechanisms of piRNAs in mediating mammalian spermatogenesis and their applications in reproductive medicine.Cellular and molecular life sciences : CMLS · 2024Review
- Heat stress induced piRNA alterations in pachytene spermatocytes and round spermatids.Reproductive biology and endocrinology : RB&E · 2024Article
- Impact of Heat Stress on Transposable Element Expression and Derived Small RNAs in Drosophila subobscura.Genome biology and evolution · 2023Article
- A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila.Nature cell biology · 2023Article
- miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensuresScience advances · 2023Article
- Review
- TheCells · 2022Article
- Daphnia magna egg piRNA cluster expression profiles change as mothers age.BMC genomics · 2022Article
- Article
- The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis inFrontiers in molecular biosciences · 2022Article
- Transposable Element Expression and Regulation Profile in Gonads of Interspecific Hybrids ofCells · 2021Article
- A transposon expression burst accompanies the activation of Y-chromosome fertility genes during Drosophila spermatogenesis.Nature communications · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PIWI-interacting RNAs (piRNAs) are predominantly produced in animal gonads to suppress transposons during germline development. Our understanding about the piRNA biogenesis and function is predominantly from studies of the Drosophila female germline. piRNA pathway function in the male germline, however, remains poorly understood. To study overall and stage-specific features of piRNAs during spermatogenesis, we analyzed small RNAs extracted from entire wild-type testes and stage-specific arrest mutant testes enriched with spermatogonia or primary spermatocytes. We show that most active piRNA clusters in the female germline do not majorly contribute to piRNAs in testes, and abundance patterns of piRNAs mapping to different transposon families also differ between male and female germlines. piRNA production is regulated in a stage-specific manner during spermatogenesis. The piRNAs in spermatogonia-enriched testes are predominantly transposon-mapping piRNAs, and almost half of those exhibit a ping-pong signature. In contrast, the primary spermatocyte-enriched testes have a dramatically high amount of piRNAs targeting repeats like suppressor of stellate and AT-chX The transposon-mapping piRNAs in the primary spermatocyte stages lacking Argonaute3 expression also show a ping-pong signature, albeit to a lesser extent. Consistently, argonaute3 mutant testes also retain ping-pong signature-bearing piRNAs, suggesting that a noncanonical ping-pong cycle might act during spermatogenesis. Our study shows stage-specific regulation of piRNA biogenesis during spermatogenesis: An active ping-pong cycle produces abundant transposon-mapping piRNAs in spermatogonia, while in primary spermatocytes, piRNAs act to suppress the repeats and transposons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.