ArticlePlant physiology2016
Members of BTB Gene Family of Scaffold Proteins Suppress Nitrate Uptake and Nitrogen Use Efficiency.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 112 citations in OpenAlex.
- Genome-wide analyses of NPF and NRT2 reveal a potential role for a key gene, CqNRT2.2, in the quinoa response to nitrate.BMC plant biology · 2026Article
- Plant-Associated Microbiomes: Crosstalk and Engineering to Improve Nutrient Use Efficiency (NUE) in Crops of Global Importance.Plants (Basel, Switzerland) · 2026Review
- Broad complex Z2 functions as an upstream 20E-responsive regulator of the midgut chitinase gene HaCHT4 in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae).BMC genomics · 2026Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the BTB domain-Containing Protein Gene Family in Poplar.Biochemical genetics · 2026Article
- Establishment and maintenance of NRT2.1 inter-individual variability in plants.PLoS genetics · 2025Article
- Evolutionary trajectory of the NPF6.3(NRT1.1/CHL1)-centered nitrate signaling regulatory network in plant roots.BMC plant biology · 2025Article
- Omics-Based Characterization of BTB Gene Family in T. aestivum, Reveals the Potential of TaBTB11/56/57/58 in Combined Heat and Drought Stress Regulation.Rice (New York, N.Y.) · 2025Article
- Translational insights into abiotic interactions: From Arabidopsis to crop plants.The Plant cell · 2025Review
- Shining light on Arabidopsis regulatory networks integrating nitrogen use and photosynthesis.The Plant journal : for cell and molecular biology · 2025Article
- Article
- Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Boosting photosynthesis opens new opportunities for agriculture sustainability and circular economy: The BEST-CROP research and innovation action.The Plant journal : for cell and molecular biology · 2025Review
- Regulatory network of ammonium and nitrate uptake and utilization in rice.Frontiers in plant science · 2025Review
- Transcriptome and gene co-expression network analysis revealed a putative regulatory mechanism of low nitrogen response in rice seedlings.Frontiers in plant science · 2025Article
- Genome-wide identification, structural and gene expression analysis of BTB gene family in soybean.BMC plant biology · 2024Article
- The Arabidopsis SGN3/GSO1 receptor kinase integrates soil nitrogen status into shoot development.The EMBO journal · 2024Article
- Calmodulin-like protein MdCML15 interacts with MdBT2 to modulate iron homeostasis in apple.Horticulture research · 2024Article
- Transcription factor module NLP-NIGT1 fine-tunes NITRATE TRANSPORTER2.1 expression.Plant physiology · 2023Article
- Novel genes and alleles of the BTB/POZ protein family in Oryza rufipogon.Scientific reports · 2023Article
- Identification of genes associated with the regulation of cold tolerance and the RNA movement in the grafted apple.Scientific reports · 2023Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Development of crops with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. However, achieving this goal has proven difficult since NUE is a complex trait encompassing physiological and developmental processes. We thought to tackle this problem by taking a systems biology approach to identify candidate target genes. First, we used a supervised machine-learning algorithm to predict a NUE gene network in Arabidopsis (Arabidopsis thaliana). Second, we identified BT2, a member of the Bric-a-Brac/Tramtrack/Broad gene family, as the most central and connected gene in the NUE network. Third, we experimentally tested BT2 for a role in NUE. We found NUE decreased in plants overexpressing BT2 gene compared to wild-type plants under limiting nitrate conditions. In addition, NUE increased compared to wild-type plants under low nitrate conditions in double mutant plants in bt2 and its closely related homolog bt1, indicating a functional redundancy of BT1 and BT2 for NUE. Expression of the nitrate transporter genes NRT2.1 and NRT2.4 increased in the bt1/bt2 double mutant compared to wild-type plants, with a concomitant 65% increase in nitrate uptake under low nitrate conditions. Similar to Arabidopsis, we found that mutation of the BT1/BT2 ortholog gene in rice (Oryza sativa) OsBT increased NUE by 20% compared to wild-type rice plants under low nitrogen conditions. These results indicate BT gene family members act as conserved negative regulators of nitrate uptake genes and NUE in plants and highlight them as prime targets for future strategies to improve NUE in crops.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.