ArticlePlant physiology2016
Molecular Characterization of Arabidopsis GAL4/UAS Enhancer Trap Lines Identifies Novel Cell-Type-Specific Promoters.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Origin and diversity of leaf vein patterns.Journal of experimental botany · 2026Review
- Brassinosteroids control cell proliferation in the lateral root cap of the Arabidopsis root.EMBO reports · 2026Article
- Arabidopsis ribosomal RNA processing meerling mutants exhibit suspensor-derived polyembryony due to direct reprogramming of the suspensor.The Plant cell · 2024Article
- An enhancer trap system to track developmental dynamics in Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2023Article
- Distinct identities of leaf phloem cells revealed by single cell transcriptomics.The Plant cell · 2021Article
- Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal.Biodesign research · 2021Review
- Combining GAL4 GFP enhancer trap with split luciferase to measure spatiotemporal promoter activity in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Development of a Gateway-compatible two-component expression vector system for plants.Transgenic research · 2019Article
- A SOSEKI-based coordinate system interprets global polarity cues in Arabidopsis.Nature plants · 2019Article
- Molecular identification of a root apical cell-specific and stress-responsive enhancer from anPlant methods · 2019Article
- A Robust Auxin Response Network Controls Embryo and Suspensor Development through a Basic Helix Loop Helix Transcriptional Module.The Plant cell · 2019Article
- Transcriptome dynamics revealed by a gene expression atlas of the early Arabidopsis embryo.Nature plants · 2017Article
- Development of a GAL4-VP16/UAS trans-activation system for tissue specific expression in Medicago truncatula.PloS one · 2017Article
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4 authors.
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Abstract
Cell-type-specific gene expression is essential to distinguish between the numerous cell types of multicellular organism. Therefore, cell-type-specific gene expression is tightly regulated and for most genes RNA transcription is the central point of control. Thus, transcriptional reporters are broadly used markers for cell identity. In Arabidopsis (Arabidopsis thaliana), a recognized standard for cell identities is a collection of GAL4/UAS enhancer trap lines. Yet, while greatly used, very few of them have been molecularly characterized. Here, we have selected a set of 21 frequently used GAL4/UAS enhancer trap lines for detailed characterization of expression pattern and genomic insertion position. We studied their embryonic and postembryonic expression domains and grouped them into three groups (early embryo development, late embryo development, and embryonic root apical meristem lines) based on their dominant expression. We show that some of the analyzed lines are expressed in a domain often broader than the one that is reported. Additionally, we present an overview of the location of the T-DNA inserts of all lines, with one exception. Finally, we demonstrate how the obtained information can be used for generating novel cell-type-specific marker lines and for genotyping enhancer trap lines. The knowledge could therefore support the extensive use of these valuable lines.
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