Evidence map›Paper›PMID 27208298›Full record

ArticlePlant physiology2016

The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency.

Olena Poretska, Saiqi Yang, Delphine Pitorre, Wilfried Rozhon, Karin Zwerger, Marcos Castellanos Uribe, Sean May, Peter McCourt, Brigitte Poppenberger, Tobias Sieberer

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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  5. Frontiers in plant science · 2020
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  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 4 countries.

Olena PoretskaPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Saiqi YangPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Delphine PitorrePlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).ORCID 0000-0002-5480-9740
Wilfried RozhonPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Karin ZwergerPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Marcos Castellanos UribePlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Sean MayPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Peter McCourtPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).
Brigitte PoppenbergerPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.).ORCID 0000-0003-1020-0500
Tobias SiebererPlant Growth Regulation, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (O.P., S.Y., T.S.); Department for Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria (O.P., D.P., K.Z., T.S.); Biotechnology of Horticultural Crops, TUM School of Life Sciences Weihenstephan, Technische Universität München, D-85354 Freising, Germany (W.R., B.P.); Nottingham Arabidopsis Stock Centre, University of Nottingham, School of Biosciences, Sutton Bonington, LE12 5RD, UK (M.C.U., S.M.); and Cell and Systems Biology, University of Toronto, Toronto ON M5S 3B2, Canada (P.M.) tobias.sieberer@wzw.tum.de.ORCID 0000-0002-4462-9260
Technical University of Munich · DEUniversity of Vienna · ATUniversity of Nottingham · GBMax Perutz Labs · ATUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ALTERED MERISTEM PROGRAM1 (AMP1) is a member of the M28 family of carboxypeptidases with a pivotal role in plant development and stress adaptation. Its most prominent mutant defect is a unique hypertrophic shoot phenotype combining a strongly increased organ formation rate with enhanced meristem size and the formation of ectopic meristem poles. However, so far the role of AMP1 in shoot development could not be assigned to a specific molecular pathway nor is its biochemical function resolved. In this work we evaluated the level of functional conservation between AMP1 and its human homolog HsGCPII, a tumor marker of medical interest. We show that HsGCPII cannot substitute AMP1 in planta and that an HsGCPII-specific inhibitor does not evoke amp1-specific phenotypes. We used a chemical genetic approach to identify the drug hyperphyllin (HP), which specifically mimics the shoot defects of amp1, including plastochron reduction and enlargement and multiplication of the shoot meristem. We assessed the structural requirements of HP activity and excluded that it is a cytokinin analog. HP-treated wild-type plants showed amp1-related tissue-specific changes of various marker genes and a significant transcriptomic overlap with the mutant. HP was ineffective in amp1 and elevated the protein levels of PHAVOLUTA, consistent with the postulated role of AMP1 in miRNA-controlled translation, further supporting an AMP1-related mode of action. Our work suggests that plant and animal members of the M28 family of proteases adopted unrelated functions. With HP we provide a tool to characterize the plant-specific functions of this important class of proteins.

Indexed as

ArabidopsisArabidopsis ProteinsBenzamidesBiomarkersCarboxypeptidasesConserved SequenceCytokininsGene Expression Regulation, PlantHumansMeristemMicroRNAsMutationPhenotypePlant LeavesSeedlingsSequence Homology, Amino AcidAMP1 protein, ArabidopsisArabidopsis ProteinsBenzamidesBiomarkersCarboxypeptidasesCytokininshyperphyllinMicroRNAsSmall Molecule Libraries

Identifiers

PMID27208298
PMCPMC4902576
OpenAlexW2346132155

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.