ArticlePlant physiology2016
The Glycerol-3-Phosphate Acyltransferase GPAT6 from Tomato Plays a Central Role in Fruit Cutin Biosynthesis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed, 123 citations in OpenAlex.
- The novel function of the bZIP transcription factor VSF-1 in mediating fruit firmness in tomato.Horticulture research · 2026Article
- Role of the tomato MARS1/ROUGH gene encoding a LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 in adventitious root and fruit skin formation.Journal of integrative plant biology · 2026Article
- Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme.Plant biotechnology journal · 2026Article
- Barley HvBODYGUARD1 controls cuticular specialisations regulated by SHINE transcription factors.The New phytologist · 2026Article
- Advances in mutant characterization for detecting causal mutations in crop plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Targeting the tomato fruit epidermis using the nsLTP2 promoter: a molecular tool to study the composition, architecture and properties of the cuticle.Plant methods · 2026Article
- Lipid metabolism mechanisms underlying seed oil content differences in Toxicodendron vernicifluum based on transcriptomics and lipidomics.BMC plant biology · 2026Article
- Evolution of protein domains and protein domain combinations provides insights into the origin and diversification of land plants.Cell genomics · 2026Article
- Insights into glandular trichome biology from analysis of organ-specific gene expression programmes in cannabis, hop and tomato.The Plant journal : for cell and molecular biology · 2026Article
- Genome-wide characterization and transcriptional profiling of the glycerol-3-phosphate acyltransferase (GPAT) family in barley reveals the role of HvGPAT1 in low nitrogen tolerance.BMC plant biology · 2026Article
- Mitochondrial GPATs protect Arabidopsis from water stress by impacting cuticle and secondary cell wall development.Plant cell reports · 2025Article
- Review
- Naturally russeted and wound russeted skins of mango (cv. 'Apple') show no differences in anatomy, chemical composition or gene expression.Scientific reports · 2025Article
- Integrative dynamics of cell wall architecture and plant growth under salt stress.Frontiers in plant science · 2025Review
- Exploring the sheen: a review of research advances on fruit glossiness.Frontiers in plant science · 2025Review
- An InDel variant in the promoter of the NAC transcription factor MdNAC18.1 plays a major role in apple fruit ripening.The Plant cell · 2024Article
- Ribo-seq and RNA-seq analyses enrich the regulatory network of tomato fruit cracking.BMC plant biology · 2024Article
- Exploration of a European-centered strawberry diversity panel provides markers and candidate genes for the control of fruit quality traits.Horticulture research · 2024Article
- Cuticle deposition ceases during strawberry fruit development.BMC plant biology · 2024Article
- Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis ofInternational journal of molecular sciences · 2024Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micro-Tom and isolated several glossy fruit mutants in which the abundance of cutin, the polyester component of the cuticle, was strongly reduced. We employed a newly developed mapping-by-sequencing strategy to identify the causal mutation underlying the cutin deficiency in a mutant thereafter named gpat6-a (for glycerol-3-phosphate acyltransferase6). To this end, a backcross population (BC1F2) segregating for the glossy trait was phenotyped. Individuals displaying either a wild-type or a glossy fruit trait were then pooled into bulked populations and submitted to whole-genome sequencing prior to mutation frequency analysis. This revealed that the causal point mutation in the gpat6-a mutant introduces a charged amino acid adjacent to the active site of a GPAT6 enzyme. We further showed that this mutation completely abolished the GPAT activity of the recombinant protein. The gpat6-a mutant showed perturbed pollen formation but, unlike a gpat6 mutant of Arabidopsis (Arabidopsis thaliana), was not male sterile. The most striking phenotype was observed in the mutant fruit, where cuticle thickness, composition, and properties were altered. RNA sequencing analysis highlighted the main processes and pathways that were affected by the mutation at the transcriptional level, which included those associated with lipid, secondary metabolite, and cell wall biosynthesis.
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