Evidence map›Paper›PMID 27208293›Full record

ArticlePlant physiology2016

Cell Death Control by Matrix Metalloproteinases.

Dirk Zimmermann, Juan A Gomez-Barrera, Christian Pasule, Ursula B Brack-Frick, Elke Sieferer, Tim M Nicholson, Jens Pfannstiel, Annick Stintzi, Andreas Schaller

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Proteolysis in plant immunity.The Plant cell · 2024
    Review
  5. Review
  6. Article
  7. Differing Responses toFrontiers in plant science · 2022
    Article
  8. Article
  9. Article
  10. Extracellular proteolytic cascade in tomato activates immune protease Rcr3.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  11. Review
  12. Review
  13. Do proteolytic cascades exist in plants?Journal of experimental botany · 2019
    Review
  14. Article
  15. Indispensable Role of Proteases in Plant Innate Immunity.International journal of molecular sciences · 2018
    Review
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Dirk ZimmermannInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).
Juan A Gomez-BarreraInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).ORCID 0000-0001-5310-7758
Christian PasuleInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).ORCID 0000-0002-0244-193X
Ursula B Brack-FrickInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).ORCID 0000-0002-1100-7369
Elke SiefererInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).
Tim M NicholsonInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).ORCID 0000-0002-5874-7882
Jens PfannstielInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).
Annick StintziInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.).ORCID 0000-0002-6479-8184
Andreas SchallerInstitute of Plant Physiology and Biotechnology (D.Z., J.A.G.-B., C.P., E.S., T.M.N., A.St., A.Sc.) and Service Unit Mass Spectrometry (J.P.), University of Hohenheim, 70593 Stuttgart, Germany; andEidgenössisch Technische Hochschule Zürich, Institute of Plant Sciences, 8092 Zurich, Switzerland (U.B.B.-F.) andreas.schaller@uni-hohenheim.de.ORCID 0000-0001-6872-9576
University of Hohenheim · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In contrast to mammalian matrix metalloproteinases (MMPs) that play important roles in the remodeling of the extracellular matrix in animals, the proteases responsible for dynamic modifications of the plant cell wall are largely unknown. A possible involvement of MMPs was addressed by cloning and functional characterization of Sl2-MMP and Sl3-MMP from tomato (Solanum lycopersicum). The two tomato MMPs were found to resemble mammalian homologs with respect to gelatinolytic activity, substrate preference for hydrophobic amino acids on both sides of the scissile bond, and catalytic properties. In transgenic tomato seedlings silenced for Sl2/3-MMP expression, necrotic lesions were observed at the base of the hypocotyl. Cell death initiated in the epidermis and proceeded to include outer cortical cell layers. In later developmental stages, necrosis spread, covering the entire stem and extending into the leaves of MMP-silenced plants. The subtilisin-like protease P69B was identified as a substrate of Sl2- and Sl3-MMP. P69B was shown to colocalize with Sl-MMPs in the apoplast of the tomato hypocotyl, it exhibited increased stability in transgenic plants silenced for Sl-MMP activity, and it was cleaved and inactivated by Sl-MMPs in vitro. The induction of cell death in Sl2/3-MMP-silenced plants depended on P69B, indicating that Sl2- and Sl3-MMP act upstream of P69B in an extracellular proteolytic cascade that contributes to the regulation of cell death in tomato.

Indexed as

BiocatalysisCell DeathCloning, MolecularElectrophoresis, Gel, Two-DimensionalElectrophoretic Mobility Shift AssayHypocotylMatrix MetalloproteinasesPhenotypePlant ProteinsPlants, Genetically ModifiedProtein TransportProteomeProteomicsRNA InterferenceSolanum lycopersicumSubcellular FractionsMatrix MetalloproteinasesPlant ProteinsProteome

Identifiers

PMID27208293
PMCPMC4902629
OpenAlexW2418762580

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.