ArticlePlant physiology2016
The Putative O-Linked N-Acetylglucosamine Transferase SPINDLY Inhibits Class I TCP Proteolysis to Promote Sensitivity to Cytokinin.
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 53 citations in OpenAlex.
- SlSEC1- and SlSPY-mediated O-glycosylation stabilizes the transcription factor SlNOR to promote tomato fruit ripening.The Plant cell · 2026Article
- O-fucosylation affects abundance but not localization of select nucleocytoplasmic proteins in toxoplasma gondii.Glycobiology · 2025Article
- O-Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis.Nature communications · 2025Article
- A tale of two sugars: O-GlcNAc and O-fucose orchestrate growth, development, and acclimation in plants.Trends in biochemical sciences · 2025Review
- Structure-based virtual screening identifies small-molecule inhibitors of O-fucosyltransferase SPINDLY in Arabidopsis.The Plant cell · 2024Article
- O-glycosylation of the transcription factor SPATULA promotes style development in Arabidopsis.Nature plants · 2024Article
- SPINDLY mediates O-fucosylation of hundreds of proteins and sugar-dependent growth in Arabidopsis.The Plant cell · 2023Article
- SPINDLY O-fucosylates nuclear and cytoplasmic proteins involved in diverse cellular processes in plants.Plant physiology · 2023Article
- Physiological Roles and Mechanisms of Action of Class I TCP Transcription Factors.International journal of molecular sciences · 2023Review
- Advances in Research on the Regulation of Floral Development byCurrent issues in molecular biology · 2023Review
- DNA barcoding and comparative RNA-Seq analysis provide new insights into leaf formation using a novel resource of high-yieldingFrontiers in plant science · 2023Article
- Structural insights into mechanism and specificity of the plant protein O-fucosyltransferase SPINDLY.Nature communications · 2022Article
- FRUITFULL-like genes regulate flowering time and inflorescence architecture in tomato.The Plant cell · 2022Article
- Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants.Current opinion in structural biology · 2021Review
- KISS ME DEADLY F-box proteins modulate cytokinin responses by targeting the transcription factor TCP14 for degradation.Plant physiology · 2021Article
- Coordinating the morphogenesis-differentiation balance by tweaking the cytokinin-gibberellin equilibrium.PLoS genetics · 2021Article
- Biosensors: A Sneak Peek into Plant Cell's Immunity.Life (Basel, Switzerland) · 2021Review
- Article
- Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato.Plant methods · 2020Article
- Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.Molecular plant · 2020Article
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Authors and funding
10 authors at 5 institutions in 3 countries.
Evyatar SteinerInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
Sivan LivneInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
Tammy Kobinson-KatzInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
Lior TalInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
Oded Pri-TalInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
Assaf MosqunaInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).ORCID 0000-0003-0467-9287
Danuše TarkowskáInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).ORCID 0000-0003-1478-1904
Bruno MuellerInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
Petr TarkowskiInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.).
David WeissInstitute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel (E.S., S.L., T.K.-K., O.P.-T., A.M., D.W.);Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel (L.T.);Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Metabolomics, Institute of Experimental Botany, CZ-78371 Olomouc, Czech Republic (D.T.);Department of Plant and Microbial Biology, University of Zurich, Zurich-Basel Plant Science Center, 8008 Zurich, Switzerland (B.M.); andCentre of the Region Haná for Biotechnological and Agricultural Research, Central Laboratories and Research Support, Faculty of Science, Palacky University, and Department of Genetic Resources for Vegetables, Medicinal, and Special Plants, Crop Research Institute, CZ-78371 Olomouc, Czech Republic (P.T.) david.weiss@mail.huji.ac.il.ORCID 0000-0002-3253-8441
Hebrew University of Jerusalem · ILCrop Research Institute · CZCzech Academy of Sciences, Institute of Experimental Botany · CZUniversity of Zurich · CHWeizmann Institute of Science · IL
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis (Arabidopsis thaliana) SPINDLY (SPY) is a putative serine and threonine O-linked N-acetylglucosamine transferase (OGT). While SPY has been shown to suppress gibberellin signaling and to promote cytokinin (CK) responses, its catalytic OGT activity was never demonstrated and its effect on protein fate is not known. We previously showed that SPY interacts physically and functionally with TCP14 and TCP15 to promote CK responses. Here, we aimed to identify how SPY regulates TCP14/15 activities and how these TCPs promote CK responses. We show that SPY activity is required for TCP14 stability. Mutation in the putative OGT domain of SPY (spy-3) stimulated TCP14 proteolysis by the 26S proteasome, which was reversed by mutation in CULLIN1 (CUL1), suggesting a role for SKP, CUL1, F-box E3 ubiquitin ligase in TCP14 proteolysis. TCP14 proteolysis in spy-3 suppressed all TCP14 misexpression phenotypes, including the enhanced CK responses. The increased CK activity in TCP14/15-overexpressing flowers resulted from increased sensitivity to the hormone and not from higher CK levels. TCP15 overexpression enhanced the response of the CK-induced synthetic promoter pTCS to CK, suggesting that TCP14/15 affect early steps in CK signaling. We propose that posttranslational modification of TCP14/15 by SPY inhibits their proteolysis and that the accumulated proteins promote the activity of the CK phosphorelay cascade in developing Arabidopsis leaves and flowers.
Indexed as
Arabidopsis ProteinsCatalytic DomainCytokininsProteasome Endopeptidase ComplexProtein StabilityProteolysisRepressor ProteinsTranscription FactorsArabidopsis ProteinsCytokininsProteasome Endopeptidase ComplexRepressor ProteinsSPY protein, ArabidopsisTCP14 protein, ArabidopsisTCP15 protein, ArabidopsisTranscription Factors
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