ArticlePlant physiology2016
Sequence-Specific Protein Aggregation Generates Defined Protein Knockdowns in Plants.
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Functional amyloids as molecular switches: emerging roles in diverse signalling pathways.Biochemical Society transactions · 2026Review
- Overexpression inFrontiers in cellular and infection microbiology · 2025Article
- Modification of aggregation-prone regions of Arabidopsis glutamyl-tRNA reductase leads to increased stability while maintaining enzyme activity.Frontiers in plant science · 2025Article
- Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga Cyanidioschyzon merolae.Plant physiology · 2024Article
- Effect of the aggregated protein dye YAT2150 onAntimicrobial agents and chemotherapy · 2024Article
- Exploiting the aggregation propensity of beta-lactamases to design inhibitors that induce enzyme misfolding.Nature communications · 2023Article
- Two florigens and a florigen-like protein form a triple regulatory module at the shoot apical meristem to promote reproductive transitions in rice.Nature plants · 2023Article
- Exploiting the intrinsic misfolding propensity of the KRAS oncoprotein.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Heterotypic Amyloid β interactions facilitate amyloid assembly and modify amyloid structure.The EMBO journal · 2022Article
- Multi-omic analysis shows REVEILLE clock genes are involved in carbohydrate metabolism and proteasome function.Plant physiology · 2022Article
- Amyloidogenic Peptides: New Class of Antimicrobial Peptides with the Novel Mechanism of Activity.International journal of molecular sciences · 2022Review
- Brassinosteroids Mitigate Cadmium Effects in Arabidopsis Root System without Any Cooperation with Nitric Oxide.International journal of molecular sciences · 2022Article
- Glucan, Water-Dikinase 1 (GWD1), an ideal biotechnological target for potential improving yield and quality in rice.Plant biotechnology journal · 2021Article
- Synthetic Pept-Ins as a Generic Amyloid-Like Aggregation-Based Platform forBioconjugate chemistry · 2021Article
- Targeting S100B with Peptides Encoding Intrinsic Aggregation-Prone Sequence Segments.Molecules (Basel, Switzerland) · 2021Article
- Bacterial Protein Homeostasis Disruption as a Therapeutic Intervention.Frontiers in molecular biosciences · 2021Review
- Reverse engineering synthetic antiviral amyloids.Nature communications · 2020Article
- Entropic Bristles Tune the Seeding Efficiency of Prion-Nucleating Fragments.Cell reports · 2020Article
- WALTZ-DB 2.0: an updated database containing structural information of experimentally determined amyloid-forming peptides.Nucleic acids research · 2020Article
- Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis.Nature communications · 2018Article
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Authors and funding
17 authors.
Camilla BettiDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0003-3522-6440
Isabelle VanhoutteDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0003-2878-134X
Silvie CoutuerDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0002-7539-1005
Riet De RyckeDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0001-8270-7015
Kiril MishevDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0001-5849-8786
Marnik VuylstekeDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).
Stijn AesaertDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).
Debbie RombautDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).
Rodrigo GallardoDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0003-1584-3564
Frederik De SmetDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).
Jie XuDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).
Mieke Van LijsebettensDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).
Frank Van BreusegemDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0002-3147-0860
Dirk InzéDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.).ORCID 0000-0002-3217-8407
Frederic RousseauDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.) frederic.rousseau@switch.vib-kuleuven.be joost.schymkowitz@switch.vib-kuleuven.be eugenia.russinova@psb.vib-ugent.be.
Joost SchymkowitzDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.) frederic.rousseau@switch.vib-kuleuven.be joost.schymkowitz@switch.vib-kuleuven.be eugenia.russinova@psb.vib-ugent.be.ORCID 0000-0003-2020-0168
Eugenia RussinovaDepartment of Plant Systems Biology, VIB, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium (C.B., I.V., S.C., R.D.R., K.M., S.A., D.R., M.V.L., F.V.B., D.I., E.R.);Switch Laboratory, VIB, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S.);Switch Laboratory, Department of Cellular and Molecular Medicine, University of Leuven, 3000 Leuven, Belgium (R.G., F.D.S., J.X., F.R., J.S); andGnomixx, 9000 Gent, Belgium (M.V.) frederic.rousseau@switch.vib-kuleuven.be joost.schymkowitz@switch.vib-kuleuven.be eugenia.russinova@psb.vib-ugent.be.ORCID 0000-0002-0569-1977
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein aggregation is determined by short (5-15 amino acids) aggregation-prone regions (APRs) of the polypeptide sequence that self-associate in a specific manner to form β-structured inclusions. Here, we demonstrate that the sequence specificity of APRs can be exploited to selectively knock down proteins with different localization and function in plants. Synthetic aggregation-prone peptides derived from the APRs of either the negative regulators of the brassinosteroid (BR) signaling, the glycogen synthase kinase 3/Arabidopsis SHAGGY-like kinases (GSK3/ASKs), or the starch-degrading enzyme α-glucan water dikinase were designed. Stable expression of the APRs in Arabidopsis (Arabidopsis thaliana) and maize (Zea mays) induced aggregation of the target proteins, giving rise to plants displaying constitutive BR responses and increased starch content, respectively. Overall, we show that the sequence specificity of APRs can be harnessed to generate aggregation-associated phenotypes in a targeted manner in different subcellular compartments. This study points toward the potential application of induced targeted aggregation as a useful tool to knock down protein functions in plants and, especially, to generate beneficial traits in crops.
Indexed as
Gene Expression Regulation, PlantAmino Acid SequenceArabidopsisBrassinosteroidsGene ExpressionGene Knockdown TechniquesGlycogen Synthase Kinase 3Green Fluorescent ProteinsPhenotypePlant ProteinsPlants, Genetically ModifiedProtein FoldingProtein Structure, TertiaryProtein TransportSequence AlignmentSignal TransductionBrassinosteroidsGlycogen Synthase Kinase 3Green Fluorescent ProteinsPlant Proteins
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