ArticlePlant physiology2016
Enzymatic Activity of Xyloglucan Xylosyltransferase 5.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Xyloglucan xylosyltransferase stem region mediates heterodimer formation.Nature communications · 2026Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- Multiprotein Complexes of Plant Glycosyltransferases Involved in Their Function and Trafficking.Plants (Basel, Switzerland) · 2025Review
- Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties, affecting early seedling establishment.Current biology : CB · 2024Article
- Integrated Transcriptome and Proteome Analysis Reveals That Cell Wall Activity AffectsPlants (Basel, Switzerland) · 2024Article
- Using CRISPR-Cas9 Technology to Eliminate Xyloglucan in Tobacco Cell Walls and Change the Uptake and Translocation of Inorganic Arsenic.Frontiers in plant science · 2022Article
- Xyloglucan Biosynthesis: From Genes to Proteins and Their Functions.Frontiers in plant science · 2022Review
- Genome-Wide Association Mapping of Mixed Linkage (1,3;1,4)-β-Glucan and Starch Contents in Rice Whole Grain.Frontiers in plant science · 2021Article
- Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.Scientific reports · 2020Article
- Microscale thermophoresis as a powerful tool for screening glycosyltransferases involved in cell wall biosynthesis.Plant methods · 2020Article
- Article
- Metatranscriptomic and metabolite profiling reveals vertical heterogeneity within a Zygnema green algal mat from Svalbard (High Arctic).Environmental microbiology · 2019Article
- Critical Review of Plant Cell Wall Matrix Polysaccharide Glycosyltransferase Activities Verified by Heterologous Protein Expression.Frontiers in plant science · 2019Review
- A two-phase model for the non-processive biosynthesis of homogalacturonan polysaccharides by the GAUT1:GAUT7 complex.The Journal of biological chemistry · 2018Article
- Article
- Structure of xyloglucan xylosyltransferase 1 reveals simple steric rules that define biological patterns of xyloglucan polymers.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Structure of Arabidopsis thaliana FUT1 Reveals a Variant of the GT-B Class Fold and Provides Insight into Xyloglucan Fucosylation.The Plant cell · 2016Article
- Interconnections between cell wall polymers, wall mechanics, and cortical microtubules: Teasing out causes and consequences.Plant signaling & behavior · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xyloglucan, the most abundant hemicellulosic component of the primary cell wall of flowering plants, is composed of a β-(1,4)-glucan backbone decorated with d-xylosyl residues. Three xyloglucan xylosyltransferases (XXTs) participate in xyloglucan biosynthesis in Arabidopsis (Arabidopsis thaliana). Two of these, XXT1 and XXT2, have been shown to be active in vitro, whereas the catalytic activity of XXT5 has yet to be demonstrated. By optimizing XXT2 expression in a prokaryotic system and in vitro activity assay conditions, we demonstrate that nonglycosylated XXT2 lacking its cytosolic amino-terminal and transmembrane domain displays high catalytic activity. Using this optimized procedure for the expression of XXT5, we report, to our knowledge for the first time, that recombinant XXT5 shows enzymatic activity in vitro, although at a significantly slower rate than XXT1 and XXT2. Kinetic analysis showed that XXT5 has a 7-fold higher Km and 9-fold lower kcat compared with XXT1 and XXT2. Activity assays using XXT5 in combination with XXT1 or XXT2 indicate that XXT5 is not specific for their products. In addition, mutagenesis experiments showed that the in vivo function and in vitro catalytic activity of XXT5 require the aspartate-serine-aspartate motif. These results demonstrate that XXT5 is a catalytically active xylosyltransferase involved in xylosylation of the xyloglucan backbone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.