Evidence map›Paper›PMID 27208274›Full record

ArticlePlant physiology2016

Distinguishing the Roles of Thylakoid Respiratory Terminal Oxidases in the Cyanobacterium Synechocystis sp. PCC 6803.

Maria Ermakova, Tuomas Huokko, Pierre Richaud, Luca Bersanini, Christopher J Howe, David J Lea-Smith, Gilles Peltier, Yagut Allahverdiyeva

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 75 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Maria ErmakovaLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
Tuomas HuokkoLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
Pierre RichaudLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
Luca BersaniniLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
Christopher J HoweLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
David J Lea-SmithLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
Gilles PeltierLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.).
Yagut AllahverdiyevaLaboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku FI-20014, Finland (M.E., T.H., L.B., Y.A.);Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Cadarache, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Centre National de la Recherche Scientifique, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13108 Saint-Paul-lez-Durance, France (P.R., G.P.);Aix Marseille Université, Biologie Végétale et Microbiologie Environnementales, Unité Mixte de Recherche 7265, F-13284 Marseille, France (P.R., G.P.); andDepartment of Biochemistry, University of Cambridge, Cambridge, CB2 1QW United Kingdom (C.J.H., D.J.L.-S.) allahve@utu.fi.ORCID 0000-0002-9262-1757
University of Turku · FIUniversity of Cambridge · GBAix-Marseille Université · FRCommissariat à l'Énergie Atomique et aux Énergies Alternatives · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Various oxygen-utilizing electron sinks, including the soluble flavodiiron proteins (Flv1/3), and the membrane-localized respiratory terminal oxidases (RTOs), cytochrome c oxidase (Cox) and cytochrome bd quinol oxidase (Cyd), are present in the photosynthetic electron transfer chain of Synechocystis sp. PCC 6803. However, the role of individual RTOs and their relative importance compared with other electron sinks are poorly understood, particularly under light. Via membrane inlet mass spectrometry gas exchange, chlorophyll a fluorescence, P700 analysis, and inhibitor treatment of the wild type and various mutants deficient in RTOs, Flv1/3, and photosystem I, we investigated the contribution of these complexes to the alleviation of excess electrons in the photosynthetic chain. To our knowledge, for the first time, we demonstrated the activity of Cyd in oxygen uptake under light, although it was detected only upon inhibition of electron transfer at the cytochrome b6f site and in ∆flv1/3 under fluctuating light conditions, where linear electron transfer was drastically inhibited due to impaired photosystem I activity. Cox is mostly responsible for dark respiration and competes with P700 for electrons under high light. Only the ∆cox/cyd double mutant, but not single mutants, demonstrated a highly reduced plastoquinone pool in darkness and impaired gross oxygen evolution under light, indicating that thylakoid-based RTOs are able to compensate partially for each other. Thus, both electron sinks contribute to the alleviation of excess electrons under illumination: RTOs continue to function under light, operating on slower time ranges and on a limited scale, whereas Flv1/3 responds rapidly as a light-induced component and has greater capacity.

Indexed as

Electron TransportFluorescenceLightMutationOxidation-ReductionOxidoreductasesOxygenPhotosynthesisPhotosystem II Protein ComplexPhotosystem I Protein ComplexPlastoquinoneSynechocystisThylakoidsOxidoreductasesOxygenPhotosystem II Protein ComplexPhotosystem I Protein ComplexPlastoquinone

Identifiers

PMID27208274
PMCPMC4902628
OpenAlexW2342091713

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