ArticlePlant physiology2016
An Improved Variant of Soybean Type 1 Diacylglycerol Acyltransferase Increases the Oil Content and Decreases the Soluble Carbohydrate Content of Soybeans.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Breeding Climate-Resilient Soybeans for 2050 and Beyond: Leveraging Novel Technologies to Mitigate Yield Stagnation and Climate Change Impacts.Plants (Basel, Switzerland) · 2026Review
- Genome-Wide Identification of Candidate Loci Associated with the Regulation of the Protein, Oil, and Carbohydrate Content in Soybean.Plants (Basel, Switzerland) · 2026Article
- Design of High-Diacylglycerol and Lecithin Soybean Seed Oil Using GmPDATs and GmDGATs Knockout via a CRISPR-Cas9 System.Plant biotechnology journal · 2026Article
- Targets and strategies to design soybean seed composition traits.The plant genome · 2025Review
- Article
- A Novel Soybean Diacylglycerol Acyltransferase 1b Variant with Three Amino Acid Substitutions Increases Seed Oil Content.Plant & cell physiology · 2024Article
- Comparison of grain traits and genetic diversity between Chinese and Uruguayan soybeans (Frontiers in plant science · 2024Article
- Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement.Plant biotechnology journal · 2023Article
- Characterization of Auxenochlorella protothecoides acyltransferases and potential of their protein interactions to promote the enrichment of oleic acid.Biotechnology for biofuels and bioproducts · 2023Article
- A multi-locus linear mixed model methodology for detecting small-effect QTLs for quantitative traits in MAGIC, NAM, and ROAM populations.Computational and structural biotechnology journal · 2023Article
- Identification of GmGPATs and their effect on glycerolipid biosynthesis through seed-specific expression in soybean.Molecular biology reports · 2022Article
- Expression of AtWRI1 and AtDGAT1 during soybean embryo development influences oil and carbohydrate metabolism.Plant biotechnology journal · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- An alternative angiosperm DGAT1 topology and potential motifs in the N-terminus.Frontiers in plant science · 2022Article
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition.Scientific reports · 2021Article
- A predicted transmembrane region in plant diacylglycerol acyltransferase 2 regulates specificity toward very-long-chain acyl-CoAs.The Journal of biological chemistry · 2020Article
- Quantitative trait loci analysis of seed oil content and composition of wild and cultivated soybean.BMC plant biology · 2020Article
- Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetically improved diacylglycerol acyltransferase (DGAT) variants were created to favorably alter carbon partitioning in soybean (Glycine max) seeds. Initially, variants of a type 1 DGAT from a high-oil, high-oleic acid plant seed, Corylus americana, were screened for high oil content in Saccharomyces cerevisiae Nearly all DGAT variants examined from high-oil strains had increased affinity for oleoyl-CoA, with S0.5 values decreased as much as 4.7-fold compared with the wild-type value of 0.94 µm Improved soybean DGAT variants were then designed to include amino acid substitutions observed in promising C. americana DGAT variants. The expression of soybean and C. americana DGAT variants in soybean somatic embryos resulted in oil contents as high as 10% and 12%, respectively, compared with only 5% and 7.6% oil achieved by overexpressing the corresponding wild-type DGATs. The affinity for oleoyl-CoA correlated strongly with oil content. The soybean DGAT variant that gave the greatest oil increase contained 14 amino acid substitutions out of a total of 504 (97% sequence identity with native). Seed-preferred expression of this soybean DGAT1 variant increased oil content of soybean seeds by an average of 3% (16% relative increase) in highly replicated, single-location field trials. The DGAT transgenes significantly reduced the soluble carbohydrate content of mature seeds and increased the seed protein content of some events. This study demonstrated that engineering of the native DGAT enzyme is an effective strategy to improve the oil content and value of soybeans.
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Registered trials
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