Evidence map›Paper›PMID 27208249›Full record

ArticlePlant physiology2016

Analysis of Chromatin Regulators Reveals Specific Features of Rice DNA Methylation Pathways.

Feng Tan, Chao Zhou, Qiangwei Zhou, Shaoli Zhou, Wenjing Yang, Yu Zhao, Guoliang Li, Dao-Xiu Zhou

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
23.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 148 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. DNA Methylation in Rice: Mechanisms, Regulatory Roles, and Beyond.International journal of molecular sciences · 2025
    Review
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  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
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11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Feng TanNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).
Chao ZhouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).
Qiangwei ZhouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).
Shaoli ZhouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).ORCID 0000-0001-9111-1598
Wenjing YangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).
Yu ZhaoNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).ORCID 0000-0003-4451-9907
Guoliang LiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.).
Dao-Xiu ZhouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China (F.T., C.Z., Q.Z., S.Z., W.Y., Y.Z., G.L., D.-X.Z.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Saclay, Université Paris-sud 11, 91405 Orsay, France (D.-X.Z.) dao-xiu.zhou@u-psud.fr.ORCID 0000-0002-1540-0598
Huazhong Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant DNA methylation that occurs at CG, CHG, and CHH sites (H = A, C, or T) is a hallmark of the repression of repetitive sequences and transposable elements (TEs). The rice (Oryza sativa) genome contains about 40% repetitive sequence and TEs and displays specific patterns of genome-wide DNA methylation. The mechanism responsible for the specific methylation patterns is unclear. Here, we analyzed the function of OsDDM1 (Deficient in DNA Methylation 1) and OsDRM2 (Deficient in DNA Methylation 1) in genome-wide DNA methylation, TE repression, small RNA accumulation, and gene expression. We show that OsDDM1 is essential for high levels of methylation at CHG and, to a lesser extent, CG sites in heterochromatic regions and also is required for CHH methylation that mainly locates in the genic regions of the genome. In addition to a large member of TEs, loss of OsDDM1 leads to hypomethylation and up-regulation of many protein-coding genes, producing very severe growth phenotypes at the initial generation. Importantly, we show that OsDRM2 mutation results in a nearly complete loss of CHH methylation and derepression of mainly small TE-associated genes and that OsDDM1 is involved in facilitating OsDRM2-mediated CHH methylation. Thus, the function of OsDDM1 and OsDRM2 defines distinct DNA methylation pathways in the bulk of DNA methylation of the genome, which is possibly related to the dispersed heterochromatin across chromosomes in rice and suggests that DNA methylation mechanisms may vary among different plant species.

Indexed as

DNA MethylationChromatinChromatin ImmunoprecipitationDNA Transposable ElementsGene Expression Regulation, PlantGenome, PlantGenome-Wide Association StudyHeterochromatinMutationOryzaPlant ProteinsChromatinDNA Transposable ElementsHeterochromatinPlant Proteins

Identifiers

PMID27208249
PMCPMC4936571
OpenAlexW2460353198

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.