ArticlePlant physiology2016
The Peroxisomal NAD Carrier from Arabidopsis Imports NAD in Exchange with AMP.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Metabolic Profile ofPlants (Basel, Switzerland) · 2026Article
- The NAD salvage pathway enzyme NMNAT-C sustains dark-phase NAD+ homeostasis in cyanobacteria.Plant physiology · 2026Article
- NAD(H) and NADP(H) in plants and mammals.Molecular plant · 2025Review
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- NADNature communications · 2023Article
- Peroxisomal NAD(H) Homeostasis in the YeastBiomolecules · 2023Article
- Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth.Frontiers in plant science · 2023Article
- Arabidopsis nitrate-induced aspartate oxidase gene expression is necessary to maintain metabolic balance under nitrogen nutrient fluctuation.Communications biology · 2022Article
- Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte algaFrontiers in microbiology · 2022Article
- Knockdown ofInternational journal of molecular sciences · 2021Article
- Live monitoring of plant redox and energy physiology with genetically encoded biosensors.Plant physiology · 2021Review
- Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters.Frontiers in cell and developmental biology · 2021Article
- NADSignal transduction and targeted therapy · 2020Review
- Peroxisomal Cofactor Transport.Biomolecules · 2020Review
- Metabolic Roles of Plant Mitochondrial Carriers.Biomolecules · 2020Review
- Article
- Plant Mitochondrial Carriers: Molecular Gatekeepers That Help to Regulate Plant Central Carbon Metabolism.Plants (Basel, Switzerland) · 2020Review
- The mitochondrial NADThe Plant journal : for cell and molecular biology · 2019Article
- Plant peroxisomal solute transporter proteins.Journal of integrative plant biology · 2019Review
- Expression and Functional Analysis of the Propamocarb-Related GeneFrontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cofactors such as NAD, AMP, and Coenzyme A (CoA) are essential for a diverse set of reactions and pathways in the cell. Specific carrier proteins are required to distribute these cofactors to different cell compartments, including peroxisomes. We previously identified a peroxisomal transport protein in Arabidopsis (Arabidopsis thaliana) called the peroxisomal NAD carrier (PXN). When assayed in vitro, this carrier exhibits versatile transport functions, e.g. catalyzing the import of NAD or CoA, the exchange of NAD/NADH, and the export of CoA. These observations raise the question about the physiological function of PXN in plants. Here, we used Saccharomyces cerevisiae to address this question. First, we confirmed that PXN, when expressed in yeast, is active and targeted to yeast peroxisomes. Secondl, detailed uptake analyses revealed that the CoA transport function of PXN can be excluded under physiological conditions due to its low affinity for this substrate. Third, we expressed PXN in diverse mutant yeast strains and investigated the suppression of the mutant phenotypes. These studies provided strong evidences that PXN was not able to function as a CoA transporter or a redox shuttle by mediating a NAD/NADH exchange, but instead catalyzed the import of NAD into peroxisomes against AMP in intact yeast cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.