ArticlePlant physiology2016
ZEAXANTHIN EPOXIDASE Activity Potentiates Carotenoid Degradation in Maturing Seed.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- Evaluating the Combined Effects of Elevated [COPlant, cell & environment · 2026Article
- Integrated transcriptomic and metabolomic analysis suggests a potential regulatory network underlying leaf color transition in Ulmus pumila 'Jinye'.BMC plant biology · 2026Article
- Molecular and morphological signatures of drought and salinity stress inFrontiers in plant science · 2026Article
- Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant inInternational journal of molecular sciences · 2024Article
- Identifying molecular targets for modulating carotenoid accumulation in rice grains.Biochemistry and biophysics reports · 2024Article
- Article
- Unraveling transcriptomics of sorghum grain carotenoids: a step forward for biofortification.BMC genomics · 2023Article
- Resequencing of sweetpotato germplasm resources reveals key loci associated with multiple agronomic traits.Horticulture research · 2023Article
- Transcriptome analysis and identification of abscisic acid and gibberellin-related genes during seed development of alfalfa (Medicago sativa L.).BMC genomics · 2022Article
- Comparative transcriptomics of wild and commercial Citrus during early ripening reveals how domestication shaped fruit gene expression.BMC plant biology · 2022Article
- Comparative metabolomic and transcriptomic analysis reveals a coexpression network of the carotenoid metabolism pathway in the panicle of Setaria italica.BMC plant biology · 2022Article
- Map-Based Cloning and Characterization ofFrontiers in plant science · 2022Article
- Integrated transcriptome and metabolome profiling ofFrontiers in genetics · 2022Article
- Multi-strategy engineering greatly enhances provitamin A carotenoid accumulation and stability in Arabidopsis seeds.aBIOTECH · 2021Article
- Eleven biosynthetic genes explain the majority of natural variation in carotenoid levels in maize grain.The Plant cell · 2021Article
- ABA Metabolism and Homeostasis in Seed Dormancy and Germination.International journal of molecular sciences · 2021Review
- The Genetic Basis of Tomato Aroma.Genes · 2021Review
- The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants.Frontiers in plant science · 2021Review
- Advancing provitamin A biofortification in sorghum: Genome-wide association studies of grain carotenoids in global germplasm.The plant genome · 2020Article
- Natural variation reveals that OsSAP16 controls low-temperature germination in rice.Journal of experimental botany · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidation of the carotenoid biosynthetic pathway has enabled altering the composition and content of carotenoids in various plants, but to achieve desired nutritional impacts, the genetic components regulating carotenoid homeostasis in seed, the plant organ consumed in greatest abundance, must be elucidated. We used a combination of linkage mapping, genome-wide association studies (GWAS), and pathway-level analysis to identify nine loci that impact the natural variation of seed carotenoids in Arabidopsis (Arabidopsis thaliana). ZEAXANTHIN EPOXIDASE (ZEP) was the major contributor to carotenoid composition, with mutants lacking ZEP activity showing a remarkable 6-fold increase in total seed carotenoids relative to the wild type. Natural variation in ZEP gene expression during seed development was identified as the underlying mechanism for fine-tuning carotenoid composition, stability, and ultimately content in Arabidopsis seed. We previously showed that two CAROTENOID CLEAVAGE DIOXYGENASE enzymes, CCD1 and CCD4, are the primary mediators of seed carotenoid degradation, and here we demonstrate that ZEP acts as an upstream control point of carotenoid homeostasis, with ZEP-mediated epoxidation targeting carotenoids for degradation by CCD enzymes. Finally, four of the nine loci/enzymatic activities identified as underlying natural variation in Arabidopsis seed carotenoids also were identified in a recent GWAS of maize (Zea mays) kernel carotenoid variation. This first comparison of the natural variation in seed carotenoids in monocots and dicots suggests a surprising overlap in the genetic architecture of these traits between the two lineages and provides a list of likely candidates to target for selecting seed carotenoid variation in other species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.